Detailed information of TA system TA123175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2382523..2383088
• Replicon: chromosome
• Accession: NZ_CP038801
• Organism: Treponema denticola strain ATCC 700771

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E4N77_RS11225
• Protein ID: WP_253696697.1
• Coordinates: 2382750..2383088 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M2BA40
• Locus tag: E4N77_RS11220
• Protein ID: WP_002687528.1
• Coordinates: 2382523..2382756 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4N77_RS11185 (E4N77_11530)	2378145..2379131	+	987	WP_255803163.1	hypothetical protein	-
E4N77_RS11190 (E4N77_11535)	2379276..2379557	-	282	WP_255803164.1	flagellar biosynthesis anti-sigma factor FlgM	-
E4N77_RS11195 (E4N77_11540)	2379628..2380284	-	657	WP_255804654.1	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	-
E4N77_RS11200 (E4N77_11545)	2380361..2380771	+	411	WP_255803165.1	hypothetical protein	-
E4N77_RS11205 (E4N77_11550)	2380776..2381765	-	990	WP_255803166.1	asparaginase	-
E4N77_RS11210 (E4N77_11555)	2381775..2382155	-	381	WP_255803167.1	PIN domain-containing protein	-
E4N77_RS11215 (E4N77_11560)	2382155..2382349	-	195	WP_147625230.1	type II toxin-antitoxin system VapB family antitoxin	-
E4N77_RS11220 (E4N77_11565)	2382523..2382756	+	234	WP_002687528.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
E4N77_RS11225 (E4N77_11570)	2382750..2383088	+	339	WP_253696697.1	endoribonuclease MazF	Toxin
E4N77_RS11230 (E4N77_11575)	2383128..2384270	+	1143	WP_255803168.1	vWA domain-containing protein	-
E4N77_RS11235 (E4N77_11580)	2384375..2386396	+	2022	WP_255803169.1	helicase	-
E4N77_RS11240 (E4N77_11585)	2386427..2387191	+	765	WP_255803170.1	prolyl oligopeptidase family serine peptidase	-
E4N77_RS11245 (E4N77_11590)	2387202..2387630	+	429	WP_255803171.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-110)

Antitoxin

• SpoVT-AbrB domain (6-48)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12769.73 Da
• Isoelectric Point: 7.2643

>T123175 WP_253696697.1 NZ_CP038801:2382750-2383088 [Treponema denticola]
MVNSNYVPEKGDLVWLDFDPQTGHEQKGRRPAICVSHKIYNQKTGLALFCPITGHIKGYPFEIVLNKHSINGCILSDQIK
NLDWKQRNCNFIEKALSEEIDSVIANIKLMVE

Nucleotide

• Length: 339 bp

>T123175 NZ_CP038801:2382750-2383088 [Treponema denticola]
ATGGTAAATTCAAATTATGTTCCTGAAAAAGGAGATTTAGTCTGGCTCGATTTTGATCCGCAAACAGGTCATGAGCAAAA
AGGCCGCCGTCCCGCAATTTGTGTTTCCCATAAAATATATAATCAAAAAACCGGGCTTGCTTTATTCTGTCCAATTACCG
GTCATATAAAGGGTTATCCTTTTGAGATTGTGCTGAATAAACATTCAATAAACGGATGTATATTAAGTGATCAGATAAAA
AATCTTGACTGGAAGCAAAGAAATTGTAATTTTATAGAAAAAGCGTTATCTGAAGAAATAGATTCTGTTATTGCTAATAT
AAAATTAATGGTTGAATAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8829.31 Da
• Isoelectric Point: 7.0119

>AT123175 WP_002687528.1 NZ_CP038801:2382523-2382756 [Treponema denticola]
MQMVVQKWGNSLGFRIPSLWAKDNNIKKGSKVEMIIEKEKIVILPQKKSLEDMLALINEDNIHSEISTGYAVGKEEW

Nucleotide

• Length: 234 bp

>AT123175 NZ_CP038801:2382523-2382756 [Treponema denticola]
ATGCAGATGGTAGTTCAGAAATGGGGTAATAGCTTAGGCTTTAGAATTCCTTCGCTTTGGGCTAAAGACAATAATATAAA
AAAGGGCAGTAAAGTTGAAATGATTATAGAAAAAGAGAAAATTGTTATTCTTCCTCAGAAAAAATCTCTTGAGGATATGC
TTGCATTGATAAATGAAGATAATATTCATTCAGAAATTTCTACAGGATATGCAGTAGGGAAGGAAGAATGGTAA

Structures

Antitoxin

• AlphaFold DB: M2BA40