Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 1781..2328 Replicon plasmid pDW54_2
Accession NZ_CP038793
Organism Escherichia coli strain PF9285

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag E5M00_RS26830 Protein ID WP_000969996.1
Coordinates 2047..2328 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID K4JYR0
Locus tag E5M00_RS26825 Protein ID WP_000079928.1
Coordinates 1781..2050 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5M00_RS26810 1..873 + 873 WP_001536607.1 incFII family plasmid replication initiator RepA -
E5M00_RS27645 1568..1726 + 159 WP_032072263.1 hypothetical protein -
E5M00_RS26825 1781..2050 + 270 WP_000079928.1 hypothetical protein Antitoxin
E5M00_RS26830 2047..2328 + 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E5M00_RS26835 2374..3222 + 849 WP_021511698.1 3'-5' exonuclease -
E5M00_RS26850 3985..4611 + 627 WP_021511696.1 hypothetical protein -
E5M00_RS26855 4608..6134 + 1527 WP_135798831.1 ATP-dependent helicase -
E5M00_RS26860 6245..6796 - 552 WP_001760564.1 plasmid stability family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..87604 87604


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T123170 WP_000969996.1 NZ_CP038793:2047-2328 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T123170 NZ_CP038793:2047-2328 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCTGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10175.50 Da        Isoelectric Point: 7.3210

>AT123170 WP_000079928.1 NZ_CP038793:1781-2050 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT123170 NZ_CP038793:1781-2050 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCCCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A5W2J4D0

References