Detailed information of TA system TA123162

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 61252..61811
• Replicon: plasmid pDW54_1
• Accession: NZ_CP038792
• Organism: Escherichia coli strain PF9285

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: E5M00_RS26355
• Protein ID: WP_000421257.1
• Coordinates: 61252..61527 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: E5M00_RS26360
• Protein ID: WP_024187170.1
• Coordinates: 61527..61811 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5M00_RS26335	57992..58558	+	567	WP_001235698.1	recombinase family protein	-
E5M00_RS26340	58586..59296	+	711	WP_021530386.1	hypothetical protein	-
E5M00_RS26345	59334..59948	-	615	WP_021530387.1	TIR domain-containing protein	-
E5M00_RS26350	60117..60884	+	768	WP_001159649.1	DUF1883 domain-containing protein	-
E5M00_RS26355	61252..61527	-	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E5M00_RS26360	61527..61811	-	285	WP_024187170.1	ribbon-helix-helix protein, CopG family	Antitoxin
E5M00_RS26375	62898..63755	-	858	WP_000131011.1	incFII family plasmid replication initiator RepA	-
E5M00_RS26380	63748..63822	-	75	WP_001365705.1	RepA leader peptide Tap	-
E5M00_RS26390	64069..64317	-	249	WP_021512942.1	replication regulatory protein RepA	-
E5M00_RS26395	64601..64807	-	207	WP_047649939.1	type I toxin-antitoxin system Hok family toxin	-
-	64794..64855	+	62	NuclAT_0	-	-
-	64794..64855	+	62	NuclAT_0	-	-
-	64794..64855	+	62	NuclAT_0	-	-
-	64794..64855	+	62	NuclAT_0	-	-
E5M00_RS26405	65030..65412	-	383	Protein_74	hypothetical protein	-
E5M00_RS26410	65515..65976	-	462	WP_000760078.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	espP / faeJ / faeJ / faeI / faeH / faeF / faeE / faeD / faeC / iutA / iucD / iucC / iucB / iucA	1..129920	129920

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T123162 WP_000421257.1 NZ_CP038792:c61527-61252 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T123162 NZ_CP038792:c61527-61252 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10506.98 Da
• Isoelectric Point: 5.6558

>AT123162 WP_024187170.1 NZ_CP038792:c61811-61527 [Escherichia coli]
MQMKNNTAQATKVITAHVPLLMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNLLPVPR

Nucleotide

• Length: 285 bp

>AT123162 NZ_CP038792:c61811-61527 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACTTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCTGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: S1PMX5