Detailed information of TA system TA123160

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4732278..4733110
• Replicon: chromosome
• Accession: NZ_CP038791
• Organism: Escherichia coli strain PF9285

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2X9SFA4
• Locus tag: E5M00_RS23780
• Protein ID: WP_001094442.1
• Coordinates: 4732736..4733110 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2Y0FLC6
• Locus tag: E5M00_RS23775
• Protein ID: WP_021525985.1
• Coordinates: 4732278..4732646 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5M00_RS23740	4728169..4728849	+	681	WP_001282919.1	WYL domain-containing protein	-
E5M00_RS23745	4728997..4729674	+	678	WP_001097305.1	hypothetical protein	-
E5M00_RS23750	4729680..4729913	+	234	WP_001278287.1	DUF905 family protein	-
E5M00_RS23755	4730003..4730821	+	819	WP_001175169.1	DUF945 domain-containing protein	-
E5M00_RS23760	4730913..4731398	+	486	WP_000206658.1	antirestriction protein	-
E5M00_RS23765	4731414..4731890	+	477	WP_001367707.1	RadC family protein	-
E5M00_RS23770	4731977..4732198	+	222	WP_000691819.1	DUF987 domain-containing protein	-
E5M00_RS23775	4732278..4732646	+	369	WP_021525985.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
E5M00_RS23780	4732736..4733110	+	375	WP_001094442.1	TA system toxin CbtA family protein	Toxin
E5M00_RS23785	4733107..4733595	+	489	WP_000777553.1	hypothetical protein	-
E5M00_RS23790	4733607..4733804	+	198	WP_000839281.1	DUF957 domain-containing protein	-
E5M00_RS23795	4733901..4734470	+	570	WP_001290247.1	DUF4942 domain-containing protein	-
E5M00_RS23800	4735219..4736757	+	1539	WP_001187173.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14072.01 Da
• Isoelectric Point: 7.8281

>T123160 WP_001094442.1 NZ_CP038791:4732736-4733110 [Escherichia coli]
MNTLPDTHVREASHCQSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T123160 NZ_CP038791:4732736-4733110 [Escherichia coli]
ATGAACACATTACCCGATACACACGTACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13690.48 Da
• Isoelectric Point: 6.4768

>AT123160 WP_021525985.1 NZ_CP038791:4732278-4732646 [Escherichia coli]
VSDTLPGTTHPDDNNDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT123160 NZ_CP038791:4732278-4732646 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCATCCCGACGATAACAACGACCACCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A2X9SFA4

Antitoxin

• AlphaFold DB: A0A2Y0FLC6