Detailed information of TA system TA123157

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4425036..4425850
• Replicon: chromosome
• Accession: NZ_CP038791
• Organism: Escherichia coli strain PF9285

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: E5M00_RS22060
• Protein ID: WP_001054376.1
• Coordinates: 4425036..4425293 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: E5M00_RS22065
• Protein ID: WP_001309181.1
• Coordinates: 4425305..4425850 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5M00_RS22040	4420324..4421430	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
E5M00_RS22045	4421495..4422475	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
E5M00_RS22050	4423058..4424044	-	987	WP_001323209.1	hypothetical protein	-
E5M00_RS22055	4424414..4424659	+	246	Protein_4219	GNAT family N-acetyltransferase	-
E5M00_RS22060	4425036..4425293	+	258	WP_001054376.1	hypothetical protein	Toxin
E5M00_RS22065	4425305..4425850	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
E5M00_RS22070	4425906..4426652	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
E5M00_RS22075	4426821..4427039	+	219	Protein_4223	hypothetical protein	-
E5M00_RS22080	4427438..4428559	+	1122	WP_000010829.1	M42 family metallopeptidase	-
E5M00_RS22085	4428556..4428834	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
E5M00_RS22090	4428846..4430159	+	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	4414821..4441540	26719

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T123157 WP_001054376.1 NZ_CP038791:4425036-4425293 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T123157 NZ_CP038791:4425036-4425293 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT123157 WP_001309181.1 NZ_CP038791:4425305-4425850 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT123157 NZ_CP038791:4425305-4425850 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8