Detailed information of TA system TA123145

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3312140..3312666
• Replicon: chromosome
• Accession: NZ_CP038791
• Organism: Escherichia coli strain PF9285

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: E5M00_RS16465
• Protein ID: WP_000323025.1
• Coordinates: 3312140..3312427 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: E5M00_RS16470
• Protein ID: WP_000534858.1
• Coordinates: 3312427..3312666 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5M00_RS16420	3307164..3307379	-	216	WP_000839590.1	phage lysis protein EssD	-
E5M00_RS16425	3308133..3308348	-	216	WP_000066484.1	cold shock-like protein CspB	-
E5M00_RS16430	3308649..3308861	+	213	WP_000087756.1	cold shock-like protein CspF	-
E5M00_RS16435	3308916..3309005	+	90	WP_120795389.1	hypothetical protein	-
E5M00_RS16440	3309283..3310035	-	753	WP_001556615.1	antitermination protein	-
E5M00_RS16445	3310049..3311098	-	1050	WP_001265198.1	DUF968 domain-containing protein	-
E5M00_RS16450	3311100..3311378	-	279	WP_012304870.1	hypothetical protein	-
E5M00_RS16455	3311445..3311696	-	252	WP_000980994.1	hypothetical protein	-
E5M00_RS16460	3311913..3312068	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
E5M00_RS16465	3312140..3312427	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
E5M00_RS16470	3312427..3312666	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
E5M00_RS16475	3312691..3312996	+	306	WP_001326990.1	hypothetical protein	-
E5M00_RS16480	3313199..3313531	+	333	WP_001301033.1	protein FlxA	-
E5M00_RS16485	3313968..3315281	-	1314	WP_000589005.1	ISNCY family transposase	-
E5M00_RS16500	3316682..3317107	+	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
E5M00_RS16505	3317104..3317454	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3276810..3329530	52720

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T123145 WP_000323025.1 NZ_CP038791:c3312427-3312140 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T123145 NZ_CP038791:c3312427-3312140 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT123145 WP_000534858.1 NZ_CP038791:c3312666-3312427 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT123145 NZ_CP038791:c3312666-3312427 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8