123143

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3156921..3157559 Replicon chromosome
Accession NZ_CP038791
Organism Escherichia coli strain PF9285

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag E5M00_RS15680 Protein ID WP_000813794.1
Coordinates 3156921..3157097 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag E5M00_RS15685 Protein ID WP_001270286.1
Coordinates 3157143..3157559 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5M00_RS15660 3152540..3153754 - 1215 WP_001326689.1 BenE family transporter YdcO -
E5M00_RS15665 3153807..3154343 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
E5M00_RS15670 3154416..3156377 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
E5M00_RS15675 3156469..3156699 - 231 WP_000494244.1 YncJ family protein -
E5M00_RS15680 3156921..3157097 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
E5M00_RS15685 3157143..3157559 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
E5M00_RS15690 3157638..3159044 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
E5M00_RS15695 3159289..3160434 + 1146 WP_032162439.1 ABC transporter substrate-binding protein -
E5M00_RS15700 3160452..3161465 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
E5M00_RS15705 3161466..3162407 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T123143 WP_000813794.1 NZ_CP038791:3156921-3157097 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T123143 NZ_CP038791:3156921-3157097 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT123143 WP_001270286.1 NZ_CP038791:3157143-3157559 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT123143 NZ_CP038791:3157143-3157559 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References