Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2074763..2075594 Replicon chromosome
Accession NZ_CP038791
Organism Escherichia coli strain PF9285

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag E5M00_RS10210 Protein ID WP_000854814.1
Coordinates 2074763..2075137 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag E5M00_RS10215 Protein ID WP_001285584.1
Coordinates 2075226..2075594 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5M00_RS10170 2070158..2071324 + 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
E5M00_RS10175 2071443..2071916 + 474 WP_001105389.1 DNA gyrase inhibitor SbmC -
E5M00_RS10180 2072115..2073173 + 1059 WP_001200891.1 FUSC family protein -
E5M00_RS10185 2073345..2073674 + 330 WP_000450409.1 DUF496 family protein -
E5M00_RS10190 2073775..2073957 - 183 WP_001016348.1 hypothetical protein -
E5M00_RS10195 2074011..2074157 + 147 Protein_1947 transposase domain-containing protein -
E5M00_RS10200 2074446..2074559 - 114 WP_001161660.1 DUF957 domain-containing protein -
E5M00_RS10205 2074572..2074766 - 195 WP_000988600.1 hypothetical protein -
E5M00_RS10210 2074763..2075137 - 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
E5M00_RS10215 2075226..2075594 - 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
E5M00_RS10220 2075668..2075889 - 222 WP_000692323.1 DUF987 domain-containing protein -
E5M00_RS10225 2075952..2076428 - 477 WP_001186773.1 RadC family protein -
E5M00_RS10230 2076444..2076923 - 480 WP_000860076.1 antirestriction protein -
E5M00_RS10235 2077005..2077823 - 819 WP_001234629.1 DUF945 domain-containing protein -
E5M00_RS10245 2077923..2078156 - 234 WP_001119729.1 DUF905 family protein -
E5M00_RS10250 2078235..2078690 - 456 WP_000581504.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T123130 WP_000854814.1 NZ_CP038791:c2075137-2074763 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T123130 NZ_CP038791:c2075137-2074763 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT123130 WP_001285584.1 NZ_CP038791:c2075594-2075226 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT123130 NZ_CP038791:c2075594-2075226 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References