Detailed information of TA system TA123125

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 919844..920678
• Replicon: chromosome
• Accession: NZ_CP038791
• Organism: Escherichia coli strain PF9285

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2T3TJB4
• Locus tag: E5M00_RS04540
• Protein ID: WP_000854811.1
• Coordinates: 919844..920221 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2T3TJJ3
• Locus tag: E5M00_RS04545
• Protein ID: WP_001285590.1
• Coordinates: 920310..920678 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5M00_RS04520	917642..918862	+	1221	WP_000794251.1	capsular biosynthesis protein	-
E5M00_RS04525	918885..919121	-	237	WP_000986719.1	hypothetical protein	-
E5M00_RS04530	919224..919400	-	177	WP_000839288.1	DUF957 domain-containing protein	-
E5M00_RS04535	919417..919847	-	431	Protein_864	hypothetical protein	-
E5M00_RS04540	919844..920221	-	378	WP_000854811.1	type IV toxin-antitoxin system toxin CbtA	Toxin
E5M00_RS04545	920310..920678	-	369	WP_001285590.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
E5M00_RS04550	920752..920973	-	222	WP_000692345.1	DUF987 domain-containing protein	-
E5M00_RS04555	921036..921512	-	477	WP_001186727.1	RadC family protein	-
E5M00_RS04560	921528..922013	-	486	WP_000849570.1	antirestriction protein	-
E5M00_RS04565	922068..922886	-	819	WP_001234620.1	DUF945 domain-containing protein	-
E5M00_RS04575	922986..923219	-	234	WP_001119729.1	DUF905 family protein	-
E5M00_RS04580	923298..923753	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / ugd / kpsS / afaA-VIII / afaB-VIII / afaC-VIII / afaD-VIII / afaE-VIII	789038..986856	197818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14041.14 Da
• Isoelectric Point: 7.8839

>T123125 WP_000854811.1 NZ_CP038791:c920221-919844 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Nucleotide

• Length: 378 bp

>T123125 NZ_CP038791:c920221-919844 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGCTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTTCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAATATTATCCTGGGTAAGCACCCGGAGGTGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13608.50 Da
• Isoelectric Point: 6.6242

>AT123125 WP_001285590.1 NZ_CP038791:c920678-920310 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLYSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT123125 NZ_CP038791:c920678-920310 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
ACAGCAATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A2T3TJB4

Antitoxin

• AlphaFold DB: A0A2T3TJJ3