Detailed information of TA system TA123114

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 4268651..4269320
• Replicon: chromosome
• Accession: NZ_CP038662
• Organism: Serratia nematodiphila strain DH-S01

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: A0A0P0QIF8
• Locus tag: E5D60_RS20315
• Protein ID: WP_033632171.1
• Coordinates: 4268651..4269073 (-)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A8G2G4M4
• Locus tag: E5D60_RS20320
• Protein ID: WP_004931679.1
• Coordinates: 4269054..4269320 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5D60_RS20295	4264543..4265061	+	519	WP_004931683.1	flavodoxin FldB	-
E5D60_RS20300	4265096..4266460	-	1365	WP_060560181.1	cell envelope integrity protein CreD	-
E5D60_RS20305	4266541..4267959	-	1419	WP_033632173.1	two-component system sensor histidine kinase CreC	-
E5D60_RS20310	4267956..4268651	-	696	WP_033632172.1	two-component system response regulator CreB	-
E5D60_RS20315	4268651..4269073	-	423	WP_033632171.1	protein YgfX	Toxin
E5D60_RS20320	4269054..4269320	-	267	WP_004931679.1	FAD assembly factor SdhE	Antitoxin
E5D60_RS20325	4269642..4270634	+	993	WP_033632169.1	tRNA-modifying protein YgfZ	-
E5D60_RS20330	4270673..4271170	-	498	WP_004931675.1	DUF2165 domain-containing protein	-
E5D60_RS20335	4271321..4271995	-	675	WP_033632168.1	hemolysin III family protein	-
E5D60_RS20340	4272179..4272787	+	609	WP_033632167.1	HD domain-containing protein	-
E5D60_RS20345	4272828..4273754	-	927	WP_015378971.1	ribokinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 16518.60 Da
• Isoelectric Point: 10.8114

>T123114 WP_033632171.1 NZ_CP038662:c4269073-4268651 [Serratia nematodiphila]
VAQWRCDIRISWRTQLFSLLTHGVLILLILISPWPEGFGPLWLVLLTLVVFQCIRSQKRIAAVQGELRLLADRRFSWHGR
EWRLAKKPWMPGYGMLLTLQPMEGKKRRRLWLASDCMSKEEWRHLRQLLLYPPAGDGEES

Nucleotide

• Length: 423 bp

>T123114 NZ_CP038662:c4269073-4268651 [Serratia nematodiphila]
GTGGCCCAGTGGCGATGTGATATCCGCATTTCCTGGCGCACCCAGCTGTTTTCACTGTTAACCCACGGCGTTCTGATCCT
GCTGATCCTGATTTCGCCCTGGCCGGAAGGCTTTGGCCCGCTCTGGCTGGTGCTGCTGACGCTGGTGGTGTTTCAGTGCA
TTCGCAGCCAGAAGCGCATCGCCGCGGTGCAGGGCGAGCTGCGGCTGCTGGCGGATCGGCGTTTCAGCTGGCACGGCCGC
GAGTGGCGACTGGCGAAAAAACCGTGGATGCCGGGTTACGGCATGCTGTTGACGCTGCAGCCGATGGAAGGCAAAAAGCG
CCGCCGGCTGTGGCTGGCTTCCGACTGCATGAGCAAAGAGGAATGGCGCCACCTGCGGCAGCTGTTGCTGTATCCGCCGG
CGGGTGACGGCGAGGAATCCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10387.89 Da
• Isoelectric Point: 4.7921

>AT123114 WP_004931679.1 NZ_CP038662:c4269320-4269054 [Serratia nematodiphila]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDSLNDADKALFIRLLECDDPDLFNWLMNHGAPQDGELQRMVTLIQTRN
KDRGPVAM

Nucleotide

• Length: 267 bp

>AT123114 NZ_CP038662:c4269320-4269054 [Serratia nematodiphila]
ATGGATATTAACAACAAAGCACGTATTCACTGGGCTTGCCGCCGTGGCATGCGCGAGCTGGACATTTCCATCATGCCGTT
CTTCGAATACGAATACGACAGCCTGAATGACGCGGACAAGGCGCTGTTTATCCGCCTGCTGGAGTGCGACGATCCCGATT
TGTTCAACTGGCTGATGAATCATGGCGCGCCGCAGGACGGCGAGCTGCAGAGAATGGTGACCCTGATCCAAACGCGAAAT
AAAGACCGTGGCCCAGTGGCGATGTGA

Structures

Toxin

• AlphaFold DB: A0A0P0QIF8