Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 3089302..3089930 Replicon chromosome
Accession NZ_CP038662
Organism Serratia nematodiphila strain DH-S01

Toxin (Protein)


Gene name hicA Uniprot ID A0A0P0QK57
Locus tag E5D60_RS14755 Protein ID WP_015378230.1
Coordinates 3089302..3089505 (+) Length 68 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A086GDG8
Locus tag E5D60_RS14760 Protein ID WP_015378231.1
Coordinates 3089517..3089930 (+) Length 138 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5D60_RS14750 3085167..3089138 + 3972 WP_033632771.1 trifunctional transcriptional regulator/proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase -
E5D60_RS14755 3089302..3089505 + 204 WP_015378230.1 type II toxin-antitoxin system HicA family toxin Toxin
E5D60_RS14760 3089517..3089930 + 414 WP_015378231.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
E5D60_RS14765 3089960..3090712 - 753 WP_033632770.1 L-cystine ABC transporter ATP-binding protein YecC -
E5D60_RS14770 3090716..3091378 - 663 WP_015378233.1 cystine ABC transporter permease -
E5D60_RS14775 3091378..3092178 - 801 WP_135638309.1 cystine ABC transporter substrate-binding protein -
E5D60_RS14780 3092294..3093286 - 993 WP_135638311.1 D-cysteine desulfhydrase -
E5D60_RS14785 3093411..3093920 - 510 WP_004934751.1 flagella biosynthesis regulatory protein FliZ -
E5D60_RS14790 3093975..3094697 - 723 WP_004934752.1 RNA polymerase sigma factor FliA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - fliZ / fliA / fliS 3089302..3098042 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 7523.89 Da        Isoelectric Point: 11.1475

>T123109 WP_015378230.1 NZ_CP038662:3089302-3089505 [Serratia nematodiphila]
VKSSELIALLESHGWVLRRVKGSHHQFKHPDFALIITVPHPKKDLKLGTLRQILKDAGLSALHIATR

Download         Length: 204 bp

>T123109 NZ_CP038662:3089302-3089505 [Serratia nematodiphila]
GTGAAGAGTTCAGAACTGATTGCGCTGCTGGAAAGCCACGGTTGGGTATTGCGGCGGGTAAAAGGCAGCCACCATCAATT
CAAACATCCCGATTTTGCGCTGATCATCACCGTGCCGCATCCAAAGAAAGATCTCAAGCTGGGCACGCTGCGGCAGATAC
TTAAAGACGCGGGGTTAAGTGCGCTTCACATCGCCACGCGTTAA

Antitoxin


Download         Length: 138 a.a.        Molecular weight: 14888.63 Da        Isoelectric Point: 5.4867

>AT123109 WP_015378231.1 NZ_CP038662:3089517-3089930 [Serratia nematodiphila]
MFYPAYIHTESNGSASGFFPDVPGCYFAGTSLDDAFADAQSALDAHFELLSEDNQPLPRPHAVAAHLAKDAGSFEGGQWL
LVAINMDKFDGRAERINITLPHRLLSRIDSLVRQHPGYGSRSGFLAAAARNELLKAE

Download         Length: 414 bp

>AT123109 NZ_CP038662:3089517-3089930 [Serratia nematodiphila]
ATGTTTTATCCCGCTTATATCCATACAGAATCAAACGGCAGCGCCAGCGGTTTCTTTCCCGACGTCCCTGGCTGCTACTT
CGCCGGCACCAGCCTGGACGACGCTTTTGCCGATGCCCAAAGCGCACTGGATGCGCACTTTGAGTTGTTGAGCGAAGACA
ATCAACCGCTACCGCGCCCGCATGCCGTCGCAGCCCATCTTGCCAAAGATGCGGGATCCTTCGAGGGAGGACAATGGCTA
TTGGTCGCTATCAATATGGATAAATTTGACGGCCGAGCCGAACGGATTAACATCACGTTGCCCCATCGCCTGTTAAGTCG
CATTGACTCTCTGGTTCGGCAACATCCGGGATACGGCAGCCGCAGCGGATTTCTCGCCGCCGCCGCGCGCAATGAATTGT
TGAAAGCAGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0P0QK57

Antitoxin

Source ID Structure
AlphaFold DB A0A086GDG8

References