Detailed information of TA system TA123091

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 580398..580982
• Replicon: chromosome
• Accession: NZ_CP038644
• Organism: Acinetobacter baumannii strain ACN21

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: E5D09_RS02790
• Protein ID: WP_000286964.1
• Coordinates: 580398..580700 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: E5D09_RS02795
• Protein ID: WP_000985609.1
• Coordinates: 580701..580982 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5D09_RS02750	576367..576738	-	372	Protein_542	acyl-CoA dehydrogenase	-
E5D09_RS02760	576802..577506	+	705	WP_071543226.1	IS6-like element IS1006 family transposase	-
E5D09_RS02765	577634..577972	-	339	WP_032073153.1	hypothetical protein	-
E5D09_RS02770	578031..578801	+	771	WP_032073134.1	hypothetical protein	-
E5D09_RS02775	578862..579176	-	315	WP_004728120.1	BrnA antitoxin family protein	-
E5D09_RS02780	579163..579450	-	288	WP_000438825.1	BrnT family toxin	-
E5D09_RS02785	579719..580249	-	531	WP_000172759.1	hypothetical protein	-
E5D09_RS02790	580398..580700	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E5D09_RS02795	580701..580982	+	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
E5D09_RS02800	581062..581481	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
E5D09_RS02805	581644..582978	+	1335	WP_033917544.1	ISNCY family transposase	-
E5D09_RS02810	583107..583427	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
E5D09_RS02815	583420..583692	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
E5D09_RS02820	584185..585646	+	1462	Protein_555	ABC-F type ribosomal protection protein Msr(E)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	msr(E) / mph(E)	-	578031..591724	13693
	-	inside	IScluster/Tn	msr(E) / mph(E)	-	576802..588226	11424

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T123091 WP_000286964.1 NZ_CP038644:580398-580700 [Acinetobacter baumannii]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T123091 NZ_CP038644:580398-580700 [Acinetobacter baumannii]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAGCAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAGCCGGTCGGTGAAGGTGTTTCTGAATTACGTTTCT
TCTTTGGGCCAGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTTGTTATTCTTTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT123091 WP_000985609.1 NZ_CP038644:580701-580982 [Acinetobacter baumannii]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT123091 NZ_CP038644:580701-580982 [Acinetobacter baumannii]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAGGATGATATGCAGGCTTACTTACAAGCATG
TATTGAAGAATCTAATGGCGATGCCGCATTCATTGCAAAGGCTTTAGGTAACATTGCAAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGCTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94