Detailed information of TA system TA123090

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 578862..579450
• Replicon: chromosome
• Accession: NZ_CP038644
• Organism: Acinetobacter baumannii strain ACN21

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: N8SM64
• Locus tag: E5D09_RS02780
• Protein ID: WP_000438825.1
• Coordinates: 579163..579450 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: D3JXQ4
• Locus tag: E5D09_RS02775
• Protein ID: WP_004728120.1
• Coordinates: 578862..579176 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5D09_RS02740	574309..574977	-	669	WP_029424166.1	3-oxoacid CoA-transferase subunit A	-
E5D09_RS02745	575025..576308	-	1284	WP_002011956.1	MFS transporter	-
E5D09_RS02750	576367..576738	-	372	Protein_542	acyl-CoA dehydrogenase	-
E5D09_RS02760	576802..577506	+	705	WP_071543226.1	IS6-like element IS1006 family transposase	-
E5D09_RS02765	577634..577972	-	339	WP_032073153.1	hypothetical protein	-
E5D09_RS02770	578031..578801	+	771	WP_032073134.1	hypothetical protein	-
E5D09_RS02775	578862..579176	-	315	WP_004728120.1	BrnA antitoxin family protein	Antitoxin
E5D09_RS02780	579163..579450	-	288	WP_000438825.1	BrnT family toxin	Toxin
E5D09_RS02785	579719..580249	-	531	WP_000172759.1	hypothetical protein	-
E5D09_RS02790	580398..580700	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
E5D09_RS02795	580701..580982	+	282	WP_000985609.1	putative addiction module antidote protein	-
E5D09_RS02800	581062..581481	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
E5D09_RS02805	581644..582978	+	1335	WP_033917544.1	ISNCY family transposase	-
E5D09_RS02810	583107..583427	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
E5D09_RS02815	583420..583692	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	msr(E) / mph(E)	-	578031..591724	13693
	-	inside	IScluster/Tn	msr(E) / mph(E)	-	576802..588226	11424

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11304.70 Da
• Isoelectric Point: 5.6205

>T123090 WP_000438825.1 NZ_CP038644:c579450-579163 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T123090 NZ_CP038644:c579450-579163 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12200.86 Da
• Isoelectric Point: 8.4628

>AT123090 WP_004728120.1 NZ_CP038644:c579176-578862 [Acinetobacter baumannii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT123090 NZ_CP038644:c579176-578862 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A0A8TVQ4

Antitoxin

• AlphaFold DB: A0A0A8TRI9