Detailed information of TA system TA123069

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2187703..2188217
• Replicon: chromosome
• Accession: NZ_CP038612
• Organism: Staphylococcus aureus strain O268

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: -
• Locus tag: SaO268_RS11425
• Protein ID: WP_000581796.1
• Coordinates: 2187703..2187912 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: SaO268_RS11430
• Protein ID: WP_000138932.1
• Coordinates: 2187924..2188217 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SaO268_RS11405	2183208..2184566	-	1359	WP_000611455.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
SaO268_RS11410	2184581..2185651	-	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
SaO268_RS11415	2185969..2187171	+	1203	WP_001109939.1	rod shape-determining protein RodA	-
SaO268_RS11420	2187438..2187575	-	138	WP_000828354.1	hypothetical protein	-
SaO268_RS11425	2187703..2187912	-	210	WP_000581796.1	heavy-metal-associated domain-containing protein	Toxin
SaO268_RS11430	2187924..2188217	-	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
SaO268_RS11435	2188383..2189867	+	1485	WP_000571549.1	cardiolipin synthase	-
SaO268_RS11440	2189895..2190542	+	648	WP_001187615.1	HD domain-containing protein	-
SaO268_RS11455	2191279..2192151	-	873	WP_000725802.1	membrane protein insertase YidC	-
SaO268_RS11460	2192237..2192878	-	642	WP_000483160.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7859.77 Da
• Isoelectric Point: 4.1008

>T123069 WP_000581796.1 NZ_CP038612:c2187912-2187703 [Staphylococcus aureus]
MIHQNTIYTSGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T123069 NZ_CP038612:c2187912-2187703 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACATCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT123069 WP_000138932.1 NZ_CP038612:c2188217-2187924 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT123069 NZ_CP038612:c2188217-2187924 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CATTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Antitoxin

• AlphaFold DB: W8UW13