Detailed information of TA system TA123068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2167956..2168485
• Replicon: chromosome
• Accession: NZ_CP038612
• Organism: Staphylococcus aureus strain O268

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SaO268_RS11335
• Protein ID: WP_000621175.1
• Coordinates: 2167956..2168318 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SaO268_RS11340
• Protein ID: WP_000948331.1
• Coordinates: 2168315..2168485 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SaO268_RS11310	2164934..2165704	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
SaO268_RS11315	2165679..2166158	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
SaO268_RS11320	2166160..2166486	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SaO268_RS11325	2166605..2167606	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SaO268_RS11335	2167956..2168318	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SaO268_RS11340	2168315..2168485	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SaO268_RS11345	2168570..2169718	-	1149	WP_001281150.1	alanine racemase	-
SaO268_RS11350	2169784..2170143	-	360	WP_000581198.1	holo-ACP synthase	-
SaO268_RS11355	2170147..2170638	-	492	WP_001286982.1	PH domain-containing protein	-
SaO268_RS11360	2170631..2172208	-	1578	WP_001294643.1	PH domain-containing protein	-
SaO268_RS11365	2172201..2172680	-	480	WP_001287084.1	hypothetical protein	-
SaO268_RS11370	2172889..2173449	-	561	WP_001092415.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T123068 WP_000621175.1 NZ_CP038612:c2168318-2167956 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T123068 NZ_CP038612:c2168318-2167956 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT123068 WP_000948331.1 NZ_CP038612:c2168485-2168315 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT123068 NZ_CP038612:c2168485-2168315 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1