Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 501758..502394 Replicon chromosome
Accession NZ_CP038517
Organism Bacillus altitudinis strain SCU11

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag EQK04_RS02420 Protein ID WP_003214169.1
Coordinates 502044..502394 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W8QJ31
Locus tag EQK04_RS02415 Protein ID WP_003214273.1
Coordinates 501758..502039 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQK04_RS02395 497912..498517 - 606 WP_007496491.1 rhomboid family intramembrane serine protease -
EQK04_RS02400 498612..498977 + 366 WP_017366663.1 holo-ACP synthase -
EQK04_RS02405 499138..500154 + 1017 WP_007496489.1 outer membrane lipoprotein-sorting protein -
EQK04_RS02410 500283..501464 + 1182 WP_017366664.1 alanine racemase -
EQK04_RS02415 501758..502039 + 282 WP_003214273.1 hypothetical protein Antitoxin
EQK04_RS02420 502044..502394 + 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EQK04_RS02425 502511..503341 + 831 WP_008346908.1 RsbT co-antagonist protein RsbRA -
EQK04_RS02430 503346..503714 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
EQK04_RS02435 503717..504118 + 402 WP_003214085.1 anti-sigma regulatory factor -
EQK04_RS02440 504129..505136 + 1008 WP_008346904.1 PP2C family protein-serine/threonine phosphatase -
EQK04_RS02445 505196..505525 + 330 WP_008346902.1 anti-sigma factor antagonist -
EQK04_RS02450 505522..506010 + 489 WP_007496471.1 anti-sigma B factor RsbW -
EQK04_RS02455 505976..506764 + 789 WP_012009000.1 RNA polymerase sigma factor SigB -
EQK04_RS02460 506764..507363 + 600 WP_008346897.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T123059 WP_003214169.1 NZ_CP038517:502044-502394 [Bacillus altitudinis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T123059 NZ_CP038517:502044-502394 [Bacillus altitudinis]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCAGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGATACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAGATT
CGTACGATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10864.25 Da        Isoelectric Point: 5.2726

>AT123059 WP_003214273.1 NZ_CP038517:501758-502039 [Bacillus altitudinis]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT123059 NZ_CP038517:501758-502039 [Bacillus altitudinis]
TTGTCTGAATCCAGCGCAACAAATGAAATGAAGATTCGACTGCCGGAGGCTTTAATGTCTGAGATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A081L854

References