Detailed information of TA system TA123057

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 12745..13346
• Replicon: plasmid p28Eco12
• Accession: NZ_CP038506
• Organism: Escherichia coli strain 28Eco12

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: DC440_RS26530
• Protein ID: WP_060579179.1
• Coordinates: 12966..13346 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: DC440_RS26525
• Protein ID: WP_001190712.1
• Coordinates: 12745..12966 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DC440_RS26475	8130..8348	+	219	WP_000209147.1	DUF4014 family protein	-
DC440_RS26480	8350..8613	+	264	WP_000224233.1	hypothetical protein	-
DC440_RS26485	8624..9622	+	999	WP_060579178.1	hypothetical protein	-
DC440_RS26495	9895..10155	+	261	WP_131728164.1	hypothetical protein	-
DC440_RS26505	10516..11040	+	525	WP_000159954.1	toprim domain-containing protein	-
DC440_RS26510	11167..11523	-	357	WP_001062545.1	hypothetical protein	-
DC440_RS26515	11524..12108	-	585	WP_032272826.1	hypothetical protein	-
DC440_RS26520	12283..12672	+	390	WP_000506726.1	DNA repair protein	-
DC440_RS26525	12745..12966	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
DC440_RS26530	12966..13346	+	381	WP_060579179.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
DC440_RS26535	13351..13530	+	180	WP_060579180.1	hypothetical protein	-
DC440_RS26540	13558..14601	+	1044	WP_060579181.1	DUF968 domain-containing protein	-
DC440_RS26545	14690..15142	+	453	WP_001312282.1	late promoter-activating protein (Gp10)	-
DC440_RS26550	15229..16422	+	1194	WP_000219605.1	terminase	-
DC440_RS26555	16422..17906	+	1485	WP_000124159.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92027	92027

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13574.27 Da
• Isoelectric Point: 5.1514

>T123057 WP_060579179.1 NZ_CP038506:12966-13346 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGGATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T123057 NZ_CP038506:12966-13346 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTAGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGCGGTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT123057 WP_001190712.1 NZ_CP038506:12745-12966 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT123057 NZ_CP038506:12745-12966 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6