Detailed information of TA system TA123054

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4819230..4820062
• Replicon: chromosome
• Accession: NZ_CP038505
• Organism: Escherichia coli strain 28Eco12

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: DC440_RS23970
• Protein ID: WP_000854765.1
• Coordinates: 4819688..4820062 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: DC440_RS23965
• Protein ID: WP_001295723.1
• Coordinates: 4819230..4819598 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DC440_RS23930	4814894..4815574	+	681	WP_001282921.1	WYL domain-containing protein	-
DC440_RS23935	4815722..4816399	+	678	WP_001097302.1	hypothetical protein	-
DC440_RS23940	4816405..4816638	+	234	WP_001278293.1	DUF905 family protein	-
DC440_RS23945	4816728..4817546	+	819	WP_001175165.1	DUF945 domain-containing protein	-
DC440_RS23950	4817812..4818291	+	480	WP_057109541.1	antirestriction protein	-
DC440_RS23955	4818307..4818783	+	477	WP_001186774.1	RadC family protein	-
DC440_RS23960	4818846..4819067	+	222	WP_000692341.1	DUF987 domain-containing protein	-
DC440_RS23965	4819230..4819598	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DC440_RS23970	4819688..4820062	+	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
DC440_RS23975	4820062..4820265	+	204	WP_001398797.1	hypothetical protein	-
DC440_RS23980	4820290..4820484	+	195	WP_024181941.1	DUF957 domain-containing protein	-
DC440_RS23985	4820569..4821411	+	843	WP_022645115.1	DUF4942 domain-containing protein	-
DC440_RS23990	4822160..4823698	+	1539	WP_001187178.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4785739..4831511	45772

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T123054 WP_000854765.1 NZ_CP038505:4819688-4820062 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T123054 NZ_CP038505:4819688-4820062 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT123054 WP_001295723.1 NZ_CP038505:4819230-4819598 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT123054 NZ_CP038505:4819230-4819598 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52