Detailed information of TA system TA123050

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4489041..4489549
• Replicon: chromosome
• Accession: NZ_CP038505
• Organism: Escherichia coli strain 28Eco12

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: DC440_RS22175
• Protein ID: WP_001534843.1
• Coordinates: 4489340..4489549 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0V9L4L3
• Locus tag: DC440_RS22170
• Protein ID: WP_000063161.1
• Coordinates: 4489041..4489343 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DC440_RS22155	4485569..4487719	+	2151	WP_001366791.1	pyruvate/proton symporter BtsT	-
DC440_RS22160	4487837..4488040	+	204	WP_001534846.1	YbdD/YjiX family protein	-
DC440_RS22165	4488051..4489007	+	957	WP_001534845.1	GTPase	-
DC440_RS22170	4489041..4489343	-	303	WP_000063161.1	BrnA antitoxin family protein	Antitoxin
DC440_RS22175	4489340..4489549	-	210	WP_001534843.1	BrnT family toxin	Toxin
DC440_RS22180	4489645..4490811	+	1167	WP_001534841.1	restriction endonuclease	-
DC440_RS22185	4490875..4492494	+	1620	WP_001550561.1	type I restriction-modification system subunit M	-
DC440_RS22190	4492484..4493794	+	1311	WP_001534837.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7952.17 Da
• Isoelectric Point: 4.9493

>T123050 WP_001534843.1 NZ_CP038505:c4489549-4489340 [Escherichia coli]
MVDYPKRDAISNINAIIVDTFLYIQLGMATMPMEFEWNANKAKSNRVKHGIRFEDAVLVLDDPQRTEPL

Nucleotide

• Length: 210 bp

>T123050 NZ_CP038505:c4489549-4489340 [Escherichia coli]
ATGGTTGATTATCCAAAACGTGATGCTATTTCCAACATCAACGCTATAATTGTAGATACATTTTTGTATATACAATTAGG
GATGGCAACTATGCCAATGGAGTTTGAATGGAATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTG
AAGATGCGGTGTTAGTGCTTGATGATCCACAACGAACGGAGCCGCTATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11600.10 Da
• Isoelectric Point: 9.4646

>AT123050 WP_000063161.1 NZ_CP038505:c4489343-4489041 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKVLANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT123050 NZ_CP038505:c4489343-4489041 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGTGCTGGCGAA
CAAATTCGACGAGGATATTGATTACAGCGACATCCCATCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0V9L4L3