Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3927425..3928043 Replicon chromosome
Accession NZ_CP038505
Organism Escherichia coli strain 28Eco12

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag DC440_RS19500 Protein ID WP_001291435.1
Coordinates 3927825..3928043 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag DC440_RS19495 Protein ID WP_000344800.1
Coordinates 3927425..3927799 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DC440_RS19485 3922514..3923707 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
DC440_RS19490 3923730..3926879 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
DC440_RS19495 3927425..3927799 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
DC440_RS19500 3927825..3928043 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
DC440_RS19505 3928215..3928766 + 552 WP_000102564.1 maltose O-acetyltransferase -
DC440_RS19510 3928882..3929352 + 471 WP_000136192.1 YlaC family protein -
DC440_RS19515 3929516..3931066 + 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
DC440_RS19520 3931108..3931461 - 354 WP_001550741.1 DUF1428 family protein -
DC440_RS19530 3931840..3932151 + 312 WP_000409911.1 MGMT family protein -
DC440_RS19535 3932182..3932754 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T123047 WP_001291435.1 NZ_CP038505:3927825-3928043 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T123047 NZ_CP038505:3927825-3928043 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT123047 WP_000344800.1 NZ_CP038505:3927425-3927799 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT123047 NZ_CP038505:3927425-3927799 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References