Detailed information of TA system TA123046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3524860..3525565
• Replicon: chromosome
• Accession: NZ_CP038505
• Organism: Escherichia coli strain 28Eco12

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: DC440_RS17710
• Protein ID: WP_000539521.1
• Coordinates: 3524860..3525246 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: DC440_RS17715
• Protein ID: WP_001280945.1
• Coordinates: 3525236..3525565 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DC440_RS17690	3520864..3521490	+	627	WP_001295292.1	glutathione S-transferase GstB	-
DC440_RS17695	3521487..3522602	-	1116	WP_000555051.1	aldose sugar dehydrogenase YliI	-
DC440_RS17700	3522713..3523096	-	384	WP_000497137.1	biofilm formation regulator BssR	-
DC440_RS17705	3523309..3524634	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
DC440_RS17710	3524860..3525246	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DC440_RS17715	3525236..3525565	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
DC440_RS17720	3525635..3526963	-	1329	WP_001550887.1	GGDEF domain-containing protein	-
DC440_RS17725	3526971..3529319	-	2349	WP_001550886.1	EAL domain-containing protein	-
DC440_RS17730	3529497..3530408	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T123046 WP_000539521.1 NZ_CP038505:3524860-3525246 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T123046 NZ_CP038505:3524860-3525246 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT123046 WP_001280945.1 NZ_CP038505:3525236-3525565 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT123046 NZ_CP038505:3525236-3525565 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6