Detailed information of TA system TA123020

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 4626..5210
• Replicon: plasmid unnamed1
• Accession: NZ_CP038501
• Organism: Acinetobacter baumannii strain CIAT758

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: E4664_RS19835
• Protein ID: WP_000286964.1
• Coordinates: 4908..5210 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: E4664_RS19830
• Protein ID: WP_000985609.1
• Coordinates: 4626..4907 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4664_RS19785	460..729	+	270	WP_039048204.1	hypothetical protein	-
E4664_RS19790	776..916	+	141	Protein_2	transposase domain-containing protein	-
E4664_RS19795	1059..1379	+	321	WP_000897309.1	type II toxin-antitoxin system RelE/ParE family toxin	-
E4664_RS19800	1372..1644	+	273	WP_094069338.1	helix-turn-helix domain-containing protein	-
E4664_RS19805	2129..2899	-	771	WP_094069337.1	hypothetical protein	-
E4664_RS19810	2958..3296	+	339	WP_032073153.1	hypothetical protein	-
E4664_RS19815	3402..3674	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
E4664_RS19820	3667..3987	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
E4664_RS19825	4127..4546	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
E4664_RS19830	4626..4907	-	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
E4664_RS19835	4908..5210	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4664_RS19840	5359..5893	+	535	Protein_12	hypothetical protein	-
E4664_RS19845	5982..6296	-	315	WP_004728120.1	BrnA antitoxin family protein	-
E4664_RS19850	6283..6570	-	288	WP_000438825.1	BrnT family toxin	-
E4664_RS19855	6866..7507	-	642	WP_167850447.1	TetR/AcrR family transcriptional regulator C-terminal domain-containing protein	-
E4664_RS19860	7573..8760	+	1188	WP_087551687.1	Tet(A)/Tet(B)/Tet(C) family tetracycline efflux MFS transporter	-
E4664_RS19865	8902..9606	-	705	WP_001067784.1	IS6-like element IS1006 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(39) / aac(3)-IId / msr(E) / mph(E) / blaOXA-58	-	1..78125	78125

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T123020 WP_000286964.1 NZ_CP038501:c5210-4908 [Acinetobacter baumannii]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T123020 NZ_CP038501:c5210-4908 [Acinetobacter baumannii]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAGCAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAGCCGGTCGGTGAAGGTGTTTCTGAATTACGTTTCT
TCTTTGGGCCAGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTTGTTATTCTTTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT123020 WP_000985609.1 NZ_CP038501:c4907-4626 [Acinetobacter baumannii]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT123020 NZ_CP038501:c4907-4626 [Acinetobacter baumannii]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAGGATGATATGCAGGCTTACTTACAAGCATG
TATTGAAGAATCTAATGGCGATGCCGCATTCATTGCAAAGGCTTTAGGTAACATTGCAAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGCTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94