Detailed information of TA system TA123003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DhiAT/dnstrm_HI1420(antitoxin)
• Location: 1725555..1726308
• Replicon: chromosome
• Accession: NZ_CP038499
• Organism: Dickeya dianthicola strain LAR.16.03.LID

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: A0A3A4D4W2
• Locus tag: E4659_RS08045
• Protein ID: WP_024105250.1
• Coordinates: 1725555..1725812 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: dhiA
• Uniprot ID: A0A3A4CVJ0
• Locus tag: E4659_RS08050
• Protein ID: WP_024108578.1
• Coordinates: 1725820..1726308 (+)
• Length: 163 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4659_RS08030 (E4659_08155)	1720995..1721996	-	1002	WP_026594706.1	putative DNA binding domain-containing protein	-
E4659_RS08035 (E4659_08160)	1722332..1724482	-	2151	WP_024108577.1	formate dehydrogenase subunit alpha	-
E4659_RS08040 (E4659_08165)	1724517..1725059	-	543	WP_013317225.1	4Fe-4S dicluster domain-containing protein	-
E4659_RS08045 (E4659_08170)	1725555..1725812	+	258	WP_024105250.1	DUF4160 domain-containing protein	Toxin
E4659_RS08050 (E4659_08175)	1725820..1726308	+	489	WP_024108578.1	addiction module antitoxin	Antitoxin
E4659_RS08055 (E4659_08180)	1726443..1726940	-	498	WP_024108579.1	hydrogenase maturation peptidase HycI	-
E4659_RS08060 (E4659_08185)	1726933..1727331	-	399	WP_024105253.1	formate hydrogenlyase maturation HycH family protein	-
E4659_RS08065 (E4659_08190)	1727328..1728119	-	792	WP_024108580.1	NADH-quinone oxidoreductase subunit B family protein	-
E4659_RS08070 (E4659_08195)	1728116..1728685	-	570	WP_024105255.1	hydrogenase 4 subunit H	-
E4659_RS08075 (E4659_08200)	1728696..1730423	-	1728	WP_024105256.1	hydrogenase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-74)

Antitoxin

• Uncharacterized protein HI1420 (80-157)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10039.68 Da
• Isoelectric Point: 8.9654

>T123003 WP_024105250.1 NZ_CP038499:1725555-1725812 [Dickeya dianthicola]
MPEIDRLAGLIFHLYFYDDERHHLPHVHVCYGHHELIISIRTGIEIEGYLPNNKRKLALVHIRAHRQQLLSMWERAVVGR
NPGKL

Nucleotide

• Length: 258 bp

>T123003 NZ_CP038499:1725555-1725812 [Dickeya dianthicola]
ATGCCTGAGATTGACAGACTGGCAGGGCTAATTTTCCATTTGTACTTTTACGATGATGAACGGCACCACTTGCCGCATGT
GCATGTTTGTTATGGCCATCATGAACTTATCATCAGTATTCGTACCGGAATCGAGATCGAAGGATATCTGCCGAATAACA
AGCGCAAGCTGGCGCTTGTGCATATCCGGGCCCACCGCCAACAGCTGTTGAGCATGTGGGAACGGGCGGTAGTGGGGAGA
AATCCGGGAAAACTGTGA

Antitoxin

Protein

• Length: 163 a.a.
• Molecular weight: 18053.72 Da
• Isoelectric Point: 8.0587

>AT123003 WP_024108578.1 NZ_CP038499:1725820-1726308 [Dickeya dianthicola]
MHKIVEVDVIGDFSLRLTYEDGLVCDINLYHYAPSPVFSNASQFIKFGLLPDGSLEWLPDIKLSASELRKKGEYVSHTLA
VRERNFADVISRAFYDAVIENRPEILQAALKIAVEKYGNSTVAKKAGAKSRTSLYKSLNKNTHVSLATVVSLAHTVLQLE
EK

Nucleotide

• Length: 489 bp

>AT123003 NZ_CP038499:1725820-1726308 [Dickeya dianthicola]
ATGCATAAAATTGTGGAAGTTGACGTCATTGGCGATTTTTCGTTGCGCCTGACCTATGAAGATGGTCTGGTTTGCGACAT
CAATCTTTATCACTATGCCCCCAGCCCGGTATTTTCGAACGCCAGCCAATTTATCAAGTTTGGGTTGCTGCCGGATGGCT
CGCTGGAATGGCTGCCGGATATTAAGCTTTCCGCGTCTGAATTGCGGAAAAAAGGGGAGTATGTCAGCCACACGCTGGCG
GTGCGAGAACGGAACTTCGCTGATGTCATCTCTCGCGCTTTCTATGATGCCGTGATTGAAAACCGGCCGGAAATTCTGCA
AGCGGCGTTGAAAATTGCGGTGGAGAAATACGGTAACAGCACGGTGGCGAAAAAAGCGGGGGCGAAAAGCCGCACCAGTC
TGTATAAGTCGCTGAATAAGAATACCCATGTCAGCCTGGCGACGGTGGTCAGCCTGGCGCATACCGTGCTGCAACTGGAA
GAGAAGTAG

Structures

Toxin

• AlphaFold DB: A0A3A4D4W2

Antitoxin

• AlphaFold DB: A0A3A4CVJ0