Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/PHD-PIN |
| Location | 3136620..3137224 | Replicon | chromosome |
| Accession | NC_000962 | ||
| Organism | Mycobacterium tuberculosis H37Rv | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | P71623 |
| Locus tag | Rv2829c | Protein ID | NP_217345.1 |
| Coordinates | 3136620..3137012 (-) | Length | 131 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | P71622 |
| Locus tag | Rv2830c | Protein ID | NP_217346.1 |
| Coordinates | 3137009..3137224 (-) | Length | 72 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Rv2824c | 3131770..3132714 | - | 945 | NP_217340.1 | CRISPR-associated endoribonuclease Cas6 | - |
| Rv2825c | 3132892..3133539 | - | 648 | NP_217341.1 | hypothetical protein | - |
| Rv2826c | 3133709..3134593 | - | 885 | NP_217342.1 | hypothetical protein | - |
| Rv2827c | 3134596..3135483 | - | 888 | NP_217343.1 | hypothetical protein | - |
| Rv2828c | 3135788..3136333 | - | 546 | NP_217344.1 | hypothetical protein | - |
| Rv2828A | 3136330..3136599 | - | 270 | YP_007411535.1 | hypothetical protein | - |
| Rv2829c | 3136620..3137012 | - | 393 | NP_217345.1 | ribonuclease VapC22 | Toxin |
| Rv2830c | 3137009..3137224 | - | 216 | NP_217346.1 | antitoxin VapB22 | Antitoxin |
| Rv2831 | 3137271..3138020 | + | 750 | NP_217347.1 | enoyl-CoA hydratase EchA16 | - |
| Rv2832c | 3138099..3139181 | - | 1083 | NP_217348.1 | sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC | - |
| Rv2833c | 3139174..3140484 | - | 1311 | NP_217349.1 | sn-glycerol-3-phosphate ABC transporter substrate-binding lipoprotein UgpB | - |
| Rv2834c | 3140487..3141314 | - | 828 | NP_217350.1 | sn-glycerol-3-phosphate ABC transporter permease UgpE | - |
| Rv2835c | 3141311..3142222 | - | 912 | NP_217351.1 | sn-glycerol-3-phosphate ABC transporter permease UgpA | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 131 a.a. Molecular weight: 14610.74 Da Isoelectric Point: 6.7594
>T123 NP_217345.1 NC_000962:c3137012-3136620 [Mycobacterium tuberculosis H37Rv]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW
Download Length: 393 bp
>T123 NC_000962:c3137012-3136620 [Mycobacterium tuberculosis H37Rv]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG
Antitoxin
Download Length: 72 a.a. Molecular weight: 7483.50 Da Isoelectric Point: 4.9732
>AT123 NP_217346.1 NC_000962:c3137224-3137009 [Mycobacterium tuberculosis H37Rv]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAADDDELFTTGVSWNVS
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAADDDELFTTGVSWNVS
Download Length: 216 bp
>AT123 NC_000962:c3137224-3137009 [Mycobacterium tuberculosis H37Rv]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGCGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGCGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113]
(2) Sakshi Agarwal et al. (2020) VapBC22 toxin-antitoxin system from Mycobacterium tuberculosis is required for pathogenesis and modulation of host immune response. Science Advances 6(23):eaba6944. [PubMed:32537511]