Detailed information of TA system TA122997

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 724258..724936
• Replicon: chromosome
• Accession: NZ_CP038499
• Organism: Dickeya dianthicola strain LAR.16.03.LID

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: E4659_RS03385
• Protein ID: WP_024104636.1
• Coordinates: 724505..724936 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A3A4CJ89
• Locus tag: E4659_RS03380
• Protein ID: WP_024104635.1
• Coordinates: 724258..724524 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4659_RS03365 (E4659_03395)	721342..721950	-	609	WP_024108232.1	HD domain-containing protein	-
E4659_RS03370 (E4659_03400)	722223..722873	+	651	WP_024104633.1	hemolysin III family protein	-
E4659_RS03375 (E4659_03405)	722964..723944	-	981	WP_024104634.1	tRNA-modifying protein YgfZ	-
E4659_RS03380 (E4659_03410)	724258..724524	+	267	WP_024104635.1	FAD assembly factor SdhE	Antitoxin
E4659_RS03385 (E4659_03415)	724505..724936	+	432	WP_024104636.1	protein YgfX	Toxin
E4659_RS03390 (E4659_03420)	725031..725549	-	519	WP_024104637.1	flavodoxin FldB	-
E4659_RS03395 (E4659_03425)	725678..727003	-	1326	WP_024104638.1	MFS transporter	-
E4659_RS03400 (E4659_03430)	727266..728165	+	900	WP_024104639.1	site-specific tyrosine recombinase XerD	-
E4659_RS03405 (E4659_03435)	728255..728971	+	717	WP_024104640.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-132)

Antitoxin

• Flavinator of succinate dehydrogenase (8-79)

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 16839.52 Da
• Isoelectric Point: 11.7737

>T122997 WP_024104636.1 NZ_CP038499:724505-724936 [Dickeya dianthicola]
VAQWQCDLRVSWRMQLFSLLTHGFLVLMILLAPWSDGYAPLWLGLVTLVVFGFVRSQRIIKSRQGEISLHGENQLHWQQR
DWQIVRRPWVMRNGVLLSLRATNGKGHQRLWLASDSMGNEEWRLLRQLLQQHPLQGTESSRHH

Nucleotide

• Length: 432 bp

>T122997 NZ_CP038499:724505-724936 [Dickeya dianthicola]
GTGGCCCAGTGGCAATGTGATTTACGCGTATCCTGGCGCATGCAGCTGTTTTCGCTGCTGACGCACGGGTTTCTGGTGCT
GATGATCCTGCTGGCGCCCTGGTCGGACGGCTATGCGCCGCTGTGGTTGGGCCTGGTGACGCTGGTGGTGTTCGGTTTCG
TGCGCAGTCAGCGCATCATCAAGTCGCGTCAGGGGGAAATTTCCCTGCATGGGGAAAACCAACTGCACTGGCAGCAGCGT
GACTGGCAGATAGTTCGGCGCCCGTGGGTAATGCGCAACGGCGTGTTGCTGTCATTGCGGGCAACGAATGGCAAAGGGCA
TCAGCGCTTGTGGCTGGCGTCAGACAGTATGGGTAACGAAGAATGGCGGCTGTTGCGCCAACTGTTGCAGCAGCATCCGT
TGCAGGGAACCGAATCCTCCCGCCATCATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10471.02 Da
• Isoelectric Point: 6.0666

>AT122997 WP_024104635.1 NZ_CP038499:724258-724524 [Dickeya dianthicola]
MDINNKSRIHWACRRGMRELDISIMPFFEHEYDTLSDDDKLHFIRLLQCDDPDLFNWLMNHGEPVDPALKRMVSLIQTRN
KDRGPVAM

Nucleotide

• Length: 267 bp

>AT122997 NZ_CP038499:724258-724524 [Dickeya dianthicola]
ATGGACATCAATAACAAATCACGTATTCACTGGGCGTGCAGACGCGGAATGCGTGAACTGGACATCTCCATCATGCCGTT
TTTCGAGCATGAATATGACACGCTGAGCGACGACGACAAGCTGCACTTCATTCGTCTGCTGCAGTGCGATGATCCCGACT
TGTTCAACTGGCTGATGAATCATGGCGAGCCGGTAGATCCGGCGCTGAAACGCATGGTTTCCCTTATTCAGACGCGAAAT
AAAGACCGTGGCCCAGTGGCAATGTGA

Structures

Antitoxin

• AlphaFold DB: A0A3A4CJ89