Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 463427..464169 Replicon chromosome
Accession NZ_CP038499
Organism Dickeya dianthicola strain LAR.16.03.LID

Toxin (Protein)


Gene name ataT Uniprot ID E0SIC1
Locus tag E4659_RS02120 Protein ID WP_013316117.1
Coordinates 463427..463906 (-) Length 160 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A3A4C8A5
Locus tag E4659_RS02125 Protein ID WP_024104363.1
Coordinates 463897..464169 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4659_RS02085 (E4659_02090) 458670..458993 + 324 WP_024104357.1 DNA-binding transcriptional regulator -
E4659_RS02090 (E4659_02095) 459289..460551 + 1263 WP_024104358.1 AAA family ATPase -
E4659_RS02095 (E4659_02100) 460551..461180 + 630 WP_024104359.1 DUF4276 family protein -
E4659_RS02100 (E4659_02105) 462105..462425 + 321 WP_024104360.1 type II toxin-antitoxin system RelE/ParE family toxin -
E4659_RS02105 (E4659_02110) 462415..462699 + 285 WP_024104361.1 helix-turn-helix transcriptional regulator -
E4659_RS02110 (E4659_02115) 462706..462852 + 147 Protein_408 transcriptional regulator -
E4659_RS02115 (E4659_02120) 462997..463398 - 402 WP_024104362.1 hypothetical protein -
E4659_RS02120 (E4659_02125) 463427..463906 - 480 WP_013316117.1 GNAT family N-acetyltransferase Toxin
E4659_RS02125 (E4659_02130) 463897..464169 - 273 WP_024104363.1 DUF1778 domain-containing protein Antitoxin
E4659_RS02130 (E4659_02135) 464659..465082 + 424 Protein_412 Arm DNA-binding domain-containing protein -
E4659_RS02140 (E4659_02145) 466005..466211 - 207 Protein_414 DUF1778 domain-containing protein -
E4659_RS02145 466282..466350 + 69 Protein_415 integrase -
E4659_RS02150 (E4659_02150) 466375..466467 + 93 Protein_416 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
E4659_RS02155 (E4659_02155) 466509..467897 + 1389 WP_024108132.1 hypothetical protein -
E4659_RS02160 (E4659_02160) 468047..468385 - 339 WP_024104367.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 448460..469661 21201
- flank IS/Tn - - 465513..465827 314


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(28-147)

Antitoxin

Vibrio phage ICP1, Orf50

(10-85)


Sequences

Toxin        


Download         Length: 160 a.a.        Molecular weight: 17564.34 Da        Isoelectric Point: 9.5367

>T122996 WP_013316117.1 NZ_CP038499:c463906-463427 [Dickeya dianthicola]
VGITAPELLLPQHAVVDFHCSEPSLNEWLKRKALKNQTLGASRTFVVCEAGTQRVVGFYALASGSIQRQVAPGAFRRNMP
DPIPVLVLGRLAVDERYQRMGIGAGLLKDAVLRSRNVAQQVGNKALLVHALSDEAKAFYQYWGFVPSEIQEHTLLLSLW

Download         Length: 480 bp

>T122996 NZ_CP038499:c463906-463427 [Dickeya dianthicola]
GTGGGAATAACGGCACCTGAATTATTGCTGCCTCAACATGCGGTTGTGGATTTTCATTGTTCAGAGCCGTCATTGAATGA
ATGGCTGAAACGCAAGGCGTTAAAAAATCAGACGCTGGGCGCTTCACGCACCTTCGTGGTGTGTGAAGCCGGTACACAGC
GTGTGGTGGGGTTCTATGCCCTCGCGTCGGGCAGCATCCAGCGTCAGGTTGCGCCGGGCGCATTCCGGCGCAATATGCCT
GACCCCATTCCTGTTCTGGTGCTCGGTAGATTAGCCGTTGATGAACGTTATCAACGTATGGGGATCGGTGCCGGGCTCCT
GAAAGATGCGGTACTCCGCTCCCGCAATGTAGCGCAGCAGGTAGGCAACAAAGCGTTATTGGTACATGCGTTATCTGATG
AGGCAAAAGCGTTCTACCAATACTGGGGTTTTGTCCCGTCAGAAATACAGGAACACACCTTATTATTATCGTTATGGTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10390.84 Da        Isoelectric Point: 4.6702

>AT122996 WP_024104363.1 NZ_CP038499:c464169-463897 [Dickeya dianthicola]
MRTETKETPINIRAKASQRELIDVAAKLLSKSRTEFILDAACREAEDVLLDQRLFLVNDEQYDAFIQVLESPVTDNPRMN
ALLNRKSPWE

Download         Length: 273 bp

>AT122996 NZ_CP038499:c464169-463897 [Dickeya dianthicola]
ATGCGAACAGAGACAAAAGAAACGCCGATTAATATTCGTGCTAAAGCATCACAGCGGGAGCTGATTGATGTCGCCGCTAA
ATTGCTGTCGAAATCAAGAACAGAGTTTATCCTTGATGCTGCCTGCCGTGAGGCTGAGGATGTGTTGTTGGATCAAAGGC
TGTTTCTGGTCAATGATGAACAATATGATGCTTTCATTCAGGTGCTGGAATCACCTGTCACAGATAATCCGCGTATGAAT
GCATTACTGAACAGGAAATCTCCGTGGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3N0G158

Antitoxin

Source ID Structure
AlphaFold DB A0A3A4C8A5

References