Detailed information of TA system TA122991

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 809011..809671
• Replicon: chromosome
• Accession: NZ_CP038498
• Organism: Pectobacterium punjabense strain SS95

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: A0A2T3W229
• Locus tag: E2566_RS03660
• Protein ID: WP_039477900.1
• Coordinates: 809258..809671 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: E2566_RS03655
• Protein ID: WP_107167852.1
• Coordinates: 809011..809277 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E2566_RS03635	804998..806002	-	1005	WP_107167856.1	LacI family DNA-binding transcriptional regulator	-
E2566_RS03640	806056..806664	-	609	WP_107167855.1	HD domain-containing protein	-
E2566_RS03645	807072..807719	+	648	WP_107167854.1	hemolysin III family protein	-
E2566_RS03650	807774..808775	-	1002	WP_107167853.1	tRNA-modifying protein YgfZ	-
E2566_RS03655	809011..809277	+	267	WP_107167852.1	FAD assembly factor SdhE	Antitoxin
E2566_RS03660	809258..809671	+	414	WP_039477900.1	protein YgfX	Toxin
E2566_RS03665	809898..810833	+	936	WP_107167851.1	23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF	-
E2566_RS03670	810899..811222	-	324	WP_107167850.1	hypothetical protein	-
E2566_RS03675	811251..811769	-	519	WP_107167849.1	flavodoxin FldB	-
E2566_RS03680	811931..813256	-	1326	WP_107167848.1	MHS family MFS transporter	-
E2566_RS03685	813568..814467	+	900	WP_107167847.1	site-specific tyrosine recombinase XerD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-132)

Antitoxin

• Flavinator of succinate dehydrogenase (8-79)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 16232.13 Da
• Isoelectric Point: 10.2792

>T122991 WP_039477900.1 NZ_CP038498:809258-809671 [Pectobacterium punjabense]
VAQWQCDLRVSWRMQLLSLVVHGLLVLLILLAPWPDGYAWLWLCLVTMVMFGFIRSQRNIKSRQGCITLLSETTLNWRQQ
EWQIVKRPWLLKNGVLLALQSADGKDKQQLWLASDSMGDDEWRHLRQLLLQQKHWAR

Nucleotide

• Length: 414 bp

>T122991 NZ_CP038498:809258-809671 [Pectobacterium punjabense]
GTGGCCCAGTGGCAATGTGACCTTCGCGTTTCCTGGCGCATGCAACTGTTGTCATTGGTTGTGCATGGCCTATTAGTGTT
GCTCATTCTGCTGGCACCGTGGCCGGACGGCTATGCGTGGCTGTGGCTCTGTCTTGTTACAATGGTGATGTTTGGTTTTA
TTCGTAGCCAAAGGAATATCAAATCGCGGCAAGGCTGTATTACTCTGCTTAGCGAAACGACCCTAAACTGGCGGCAGCAG
GAGTGGCAGATTGTCAAACGACCATGGCTTTTAAAAAATGGTGTGTTGTTGGCATTGCAGTCGGCTGATGGGAAAGACAA
GCAGCAACTGTGGCTGGCATCGGATAGCATGGGCGATGACGAATGGCGCCATTTGCGCCAACTTCTCTTACAGCAGAAAC
ACTGGGCGAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10474.86 Da
• Isoelectric Point: 5.1244

>AT122991 WP_107167852.1 NZ_CP038498:809011-809277 [Pectobacterium punjabense]
MEIDNKSRIHWACRRGMRELDIAIMPFFEHDYDTLNDSDKHTFVRLLQSDDPDLFNWLMNHGEPEDGELKRMISLIQTRN
KDRGPVAM

Nucleotide

• Length: 267 bp

>AT122991 NZ_CP038498:809011-809277 [Pectobacterium punjabense]
ATGGAAATCGATAATAAATCACGTATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTGGATATCGCCATCATGCCGTT
TTTTGAGCATGATTATGACACACTTAATGACAGTGATAAGCACACGTTTGTACGCTTGTTACAAAGCGACGACCCTGATT
TATTCAATTGGTTGATGAACCACGGTGAGCCGGAAGACGGAGAGCTGAAACGCATGATTTCCCTTATTCAGACGCGAAAT
AAAGATCGTGGCCCAGTGGCAATGTGA

Structures

Toxin

• AlphaFold DB: A0A2T3W229