Detailed information of TA system TA122986

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 176250..176867
• Replicon: chromosome
• Accession: NZ_CP038498
• Organism: Pectobacterium punjabense strain SS95

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: C6DHN3
• Locus tag: E2566_RS00795
• Protein ID: WP_010281507.1
• Coordinates: 176688..176867 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: E2566_RS00790
• Protein ID: WP_107168698.1
• Coordinates: 176250..176660 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E2566_RS00775	172168..173211	-	1044	WP_107168696.1	DNA-binding transcriptional regulator CytR	-
E2566_RS00780	173482..175680	-	2199	WP_107168697.1	primosomal protein N'	-
E2566_RS00785	175953..176168	+	216	WP_010281510.1	50S ribosomal protein L31	-
E2566_RS00790	176250..176660	-	411	WP_107168698.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
E2566_RS00795	176688..176867	-	180	WP_010281507.1	type II toxin-antitoxin system HicA family toxin	Toxin
E2566_RS00800	176951..177961	-	1011	WP_107168699.1	iron ABC transporter permease	-
E2566_RS00805	177958..178920	-	963	WP_107168700.1	ABC transporter substrate-binding protein	-
E2566_RS00810	178930..179727	-	798	WP_107168701.1	ABC transporter ATP-binding protein	-
E2566_RS00815	179945..180262	-	318	WP_005973355.1	met regulon transcriptional regulator MetJ	-
E2566_RS00820	180449..181609	+	1161	WP_039281157.1	cystathionine gamma-synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6560.69 Da
• Isoelectric Point: 10.7838

>T122986 WP_010281507.1 NZ_CP038498:c176867-176688 [Pectobacterium punjabense]
MDSRTLIAEIKADGWELIRVNGSHHHFMHPSKPGLVTIPHPKKDLPIGTVKSIRKQAGI

Nucleotide

• Length: 180 bp

>T122986 NZ_CP038498:c176867-176688 [Pectobacterium punjabense]
ATGGATAGCAGGACGTTAATAGCAGAAATCAAGGCCGATGGGTGGGAACTGATAAGAGTGAACGGAAGCCACCACCATTT
TATGCATCCTAGTAAACCGGGGTTAGTCACAATCCCCCACCCTAAGAAAGATTTACCGATAGGCACGGTAAAAAGCATCA
GGAAACAAGCAGGAATATGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14536.41 Da
• Isoelectric Point: 4.3391

>AT122986 WP_107168698.1 NZ_CP038498:c176660-176250 [Pectobacterium punjabense]
MFYPIAIEAGDDTHAYGVTVPDLPGCFSAGDTLDDAIANAKEAITGHIELLIEMGQDIPTVSSVGQLAKSAEYTGYTWAV
VDIDVTRLMGGSEKINVTLPKSLIDRIDRCVASNPEFKSRSGFLAQAALERISSSR

Nucleotide

• Length: 411 bp

>AT122986 NZ_CP038498:c176660-176250 [Pectobacterium punjabense]
ATGTTTTATCCGATTGCAATTGAAGCAGGCGACGATACCCACGCATACGGTGTAACAGTGCCAGATCTACCGGGCTGTTT
TTCTGCAGGCGATACGCTAGACGATGCCATCGCGAATGCTAAAGAGGCGATTACCGGACATATCGAACTGCTGATCGAGA
TGGGTCAGGATATTCCCACCGTGTCGTCAGTAGGCCAATTGGCAAAAAGCGCAGAATACACTGGGTACACGTGGGCGGTT
GTTGATATCGATGTGACGCGTTTAATGGGCGGTTCAGAAAAAATTAACGTCACGCTGCCTAAATCACTGATTGACCGAAT
TGATCGTTGCGTGGCGAGTAATCCTGAATTTAAAAGCCGCTCAGGTTTTCTCGCGCAGGCGGCATTAGAGCGAATCTCGT
CTTCCCGATGA

Structures

Toxin

• AlphaFold DB: A0A7W2I1S0