Detailed information of TA system TA122977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 5049911..5050665
• Replicon: chromosome
• Accession: NZ_CP038496
• Organism: Escherichia coli O157:H7 strain TT12A

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: E5F08_RS25080
• Protein ID: WP_001301452.1
• Coordinates: 5050180..5050665 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: Q8X700
• Locus tag: E5F08_RS25075
• Protein ID: WP_000801912.1
• Coordinates: 5049911..5050189 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5F08_RS25070	5047139..5049844	+	2706	WP_000906970.1	HTH-type transcriptional regulator MalT	-
E5F08_RS25075	5049911..5050189	+	279	WP_000801912.1	DUF1778 domain-containing protein	Antitoxin
E5F08_RS25080	5050180..5050665	+	486	WP_001301452.1	GNAT family N-acetyltransferase	Toxin
E5F08_RS25085	5050715..5051743	-	1029	WP_000827117.1	RNA 3'-terminal phosphate cyclase	-
E5F08_RS25090	5051747..5052973	-	1227	WP_001105473.1	RNA-splicing ligase RtcB	-
E5F08_RS25095	5053161..5054759	+	1599	WP_001232889.1	DNA-binding transcriptional regulator RtcR	-
E5F08_RS25100	5054741..5055499	-	759	WP_001302007.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17650.57 Da
• Isoelectric Point: 9.4066

>T122977 WP_001301452.1 NZ_CP038496:5050180-5050665 [Escherichia coli O157:H7]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E

Nucleotide

• Length: 486 bp

>T122977 NZ_CP038496:5050180-5050665 [Escherichia coli O157:H7]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10420.94 Da
• Isoelectric Point: 4.6336

>AT122977 WP_000801912.1 NZ_CP038496:5049911-5050189 [Escherichia coli O157:H7]
MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFILDDEQYDAFLAALDAPITAERQAK
INALMNRKSPWE

Nucleotide

• Length: 279 bp

>AT122977 NZ_CP038496:5049911-5050189 [Escherichia coli O157:H7]
ATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTCGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LHB9