Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2195098..2195893 Replicon chromosome
Accession NZ_CP038496
Organism Escherichia coli O157:H7 strain TT12A

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag E5F08_RS10330 Protein ID WP_000854914.1
Coordinates 2195519..2195893 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0B0VS41
Locus tag E5F08_RS10325 Protein ID WP_001280954.1
Coordinates 2195098..2195472 (+) Length 125 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5F08_RS10295 2190453..2191358 + 906 WP_000203541.1 chemotaxis protein -
E5F08_RS10300 2191355..2192425 + 1071 WP_000102669.1 patatin-like phospholipase family protein -
E5F08_RS10305 2192765..2193583 + 819 WP_001234682.1 DUF945 domain-containing protein -
E5F08_RS10310 2193674..2194159 + 486 WP_000214398.1 antirestriction protein -
E5F08_RS10315 2194175..2194651 + 477 WP_001186738.1 RadC family protein -
E5F08_RS10320 2194714..2194935 + 222 WP_001220314.1 DUF987 domain-containing protein -
E5F08_RS10325 2195098..2195472 + 375 WP_001280954.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
E5F08_RS10330 2195519..2195893 + 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
E5F08_RS10335 2195890..2196381 + 492 WP_000976853.1 hypothetical protein -
E5F08_RS10340 2196393..2196590 + 198 WP_000839282.1 DUF957 domain-containing protein -
E5F08_RS10345 2196675..2197517 + 843 WP_001280481.1 DUF4942 domain-containing protein -
E5F08_RS10355 2197988..2198926 + 939 WP_000351284.1 glyoxylate/hydroxypyruvate reductase GhrA -
E5F08_RS10360 2198981..2199718 + 738 WP_000283664.1 zinc-binding phosphatase -
E5F08_RS10365 2199742..2200296 + 555 WP_001001917.1 molecular chaperone YcdY -
E5F08_RS10370 2200398..2200889 + 492 WP_001301587.1 DUF1097 domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - iroB / csgG / csgF / csgE / csgD / csgB / csgA / csgC 2164371..2206457 42086


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(18-117)


Sequences


Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T122962 WP_000854914.1 NZ_CP038496:2195519-2195893 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T122962 NZ_CP038496:2195519-2195893 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13718.51 Da        Isoelectric Point: 6.6249

>AT122962 WP_001280954.1 NZ_CP038496:2195098-2195472 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT122962 NZ_CP038496:2195098-2195472 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5


Antitoxin

Source ID Structure
AlphaFold DB A0A0B0VS41

References