Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 48846..49371 Replicon plasmid pO157
Accession NZ_CP038495
Organism Escherichia coli O157:H7 strain TT12B

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag E5F07_RS26270 Protein ID WP_001159868.1
Coordinates 49066..49371 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag E5F07_RS26265 Protein ID WP_000813634.1
Coordinates 48846..49064 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5F07_RS26235 44129..44359 + 231 WP_010891288.1 hypothetical protein -
E5F07_RS26240 44480..45220 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
E5F07_RS26245 45505..46482 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
E5F07_RS26250 46890..47090 - 201 WP_000708307.1 hypothetical protein -
E5F07_RS26255 47087..47707 - 621 WP_001248529.1 hypothetical protein -
E5F07_RS26260 47704..48387 - 684 WP_010891291.1 DNA-binding protein -
E5F07_RS26265 48846..49064 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
E5F07_RS26270 49066..49371 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
E5F07_RS26275 49372..50178 + 807 WP_000016989.1 site-specific integrase -
E5F07_RS26290 52076..52414 + 339 WP_071525396.1 RepB family plasmid replication initiator protein -
E5F07_RS26295 53002..54168 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - katA / espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB 1..92707 92707


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T122953 WP_001159868.1 NZ_CP038495:49066-49371 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T122953 NZ_CP038495:49066-49371 [Escherichia coli O157:H7]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT122953 WP_000813634.1 NZ_CP038495:48846-49064 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT122953 NZ_CP038495:48846-49064 [Escherichia coli O157:H7]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References