Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4976251..4976882 Replicon chromosome
Accession NZ_CP038494
Organism Escherichia coli O157:H7 strain TT12B

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag E5F07_RS24385 Protein ID WP_001259386.1
Coordinates 4976607..4976882 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag E5F07_RS24380 Protein ID WP_000593555.1
Coordinates 4976251..4976610 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5F07_RS24350 4971363..4971836 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
E5F07_RS24355 4971833..4972114 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
E5F07_RS24360 4972191..4973549 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
E5F07_RS24365 4973542..4975050 + 1509 WP_001301773.1 carbohydrate kinase -
E5F07_RS24370 4975040..4975309 + 270 WP_000933060.1 HPr family phosphocarrier protein -
E5F07_RS24375 4975341..4976201 + 861 WP_001131788.1 class II aldolase -
E5F07_RS24380 4976251..4976610 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
E5F07_RS24385 4976607..4976882 - 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
E5F07_RS24390 4976955..4978079 - 1125 WP_001301659.1 ABC transporter permease -
E5F07_RS24395 4978079..4980814 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
E5F07_RS24400 4980811..4981878 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T122949 WP_001259386.1 NZ_CP038494:c4976882-4976607 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T122949 NZ_CP038494:c4976882-4976607 [Escherichia coli O157:H7]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT122949 WP_000593555.1 NZ_CP038494:c4976610-4976251 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT122949 NZ_CP038494:c4976610-4976251 [Escherichia coli O157:H7]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References