Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4395468..4396122 Replicon chromosome
Accession NZ_CP038494
Organism Escherichia coli O157:H7 strain TT12B

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag E5F07_RS21415 Protein ID WP_000244781.1
Coordinates 4395468..4395875 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag E5F07_RS21420 Protein ID WP_000354046.1
Coordinates 4395856..4396122 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5F07_RS21395 4391425..4393158 - 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -
E5F07_RS21400 4393164..4393874 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
E5F07_RS21405 4393899..4394795 - 897 WP_000806640.1 site-specific tyrosine recombinase XerD -
E5F07_RS21410 4394907..4395428 + 522 WP_001055874.1 flavodoxin FldB -
E5F07_RS21415 4395468..4395875 - 408 WP_000244781.1 protein YgfX Toxin
E5F07_RS21420 4395856..4396122 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
E5F07_RS21425 4396365..4397345 + 981 WP_000886053.1 tRNA-modifying protein YgfZ -
E5F07_RS21430 4397422..4398081 - 660 WP_000250274.1 hemolysin III family protein -
E5F07_RS21435 4398245..4398556 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
E5F07_RS21440 4398601..4400034 + 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T122943 WP_000244781.1 NZ_CP038494:c4395875-4395468 [Escherichia coli O157:H7]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T122943 NZ_CP038494:c4395875-4395468 [Escherichia coli O157:H7]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT122943 WP_000354046.1 NZ_CP038494:c4396122-4395856 [Escherichia coli O157:H7]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT122943 NZ_CP038494:c4396122-4395856 [Escherichia coli O157:H7]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References