Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2131864..2132659 Replicon chromosome
Accession NZ_CP038494
Organism Escherichia coli O157:H7 strain TT12B

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag E5F07_RS09875 Protein ID WP_000854914.1
Coordinates 2132285..2132659 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0B0VS41
Locus tag E5F07_RS09870 Protein ID WP_001280954.1
Coordinates 2131864..2132238 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5F07_RS09840 2127219..2128124 + 906 WP_000203541.1 chemotaxis protein -
E5F07_RS09845 2128121..2129191 + 1071 WP_000102669.1 patatin-like phospholipase family protein -
E5F07_RS09850 2129531..2130349 + 819 WP_001234682.1 DUF945 domain-containing protein -
E5F07_RS09855 2130440..2130925 + 486 WP_000214398.1 antirestriction protein -
E5F07_RS09860 2130941..2131417 + 477 WP_001186738.1 RadC family protein -
E5F07_RS09865 2131480..2131701 + 222 WP_001220314.1 DUF987 domain-containing protein -
E5F07_RS09870 2131864..2132238 + 375 WP_001280954.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
E5F07_RS09875 2132285..2132659 + 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
E5F07_RS09880 2132656..2133147 + 492 WP_000976853.1 hypothetical protein -
E5F07_RS09885 2133159..2133356 + 198 WP_000839282.1 DUF957 domain-containing protein -
E5F07_RS09890 2133441..2134283 + 843 WP_001280481.1 DUF4942 domain-containing protein -
E5F07_RS09900 2134754..2135692 + 939 WP_000351284.1 glyoxylate/hydroxypyruvate reductase GhrA -
E5F07_RS09905 2135747..2136484 + 738 WP_000283664.1 zinc-binding phosphatase -
E5F07_RS09910 2136508..2137062 + 555 WP_001001917.1 molecular chaperone YcdY -
E5F07_RS09915 2137164..2137655 + 492 WP_001301587.1 DUF1097 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T122931 WP_000854914.1 NZ_CP038494:2132285-2132659 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T122931 NZ_CP038494:2132285-2132659 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13718.51 Da        Isoelectric Point: 6.6249

>AT122931 WP_001280954.1 NZ_CP038494:2131864-2132238 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT122931 NZ_CP038494:2131864-2132238 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0VS41

References