Detailed information of TA system TA122922

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/HTH_26(antitoxin)
• Location: 2926420..2927010
• Replicon: chromosome
• Accession: NZ_CP038492
• Organism: Boseongicola sp. CCM32

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: E2K80_RS14210
• Protein ID: WP_135375596.1
• Coordinates: 2926420..2926668 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: E2K80_RS14215
• Protein ID: WP_135375597.1
• Coordinates: 2926678..2927010 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E2K80_RS14195	2923515..2924273	-	759	WP_135375594.1	outer membrane beta-barrel protein	-
E2K80_RS14200	2924678..2925238	-	561	WP_135376636.1	hypothetical protein	-
E2K80_RS14205	2925388..2926374	+	987	WP_135375595.1	D-glycerate dehydrogenase	-
E2K80_RS14210	2926420..2926668	+	249	WP_135375596.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E2K80_RS14215	2926678..2927010	+	333	WP_135375597.1	helix-turn-helix transcriptional regulator	Antitoxin
E2K80_RS14220	2927064..2928461	+	1398	WP_135375598.1	glucuronate isomerase	-
E2K80_RS14225	2928458..2929840	+	1383	WP_135375599.1	mannitol dehydrogenase family protein	-
E2K80_RS14230	2930054..2930953	-	900	WP_135375600.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-78)

Antitoxin

• Cro/C1-type helix-turn-helix domain (57-108)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9344.85 Da
• Isoelectric Point: 11.6428

>T122922 WP_135375596.1 NZ_CP038492:2926420-2926668 [Boseongicola sp. CCM32]
MKQITYRPSARKTLRRMPRNTARRILAKIEEYAHAPGSQANNVTALRGRDGIRLRVGDWRVIMQDGEVLEVLKIGPRGGV
YD

Nucleotide

• Length: 249 bp

>T122922 NZ_CP038492:2926420-2926668 [Boseongicola sp. CCM32]
ATGAAGCAGATCACCTACCGCCCATCGGCGCGGAAAACACTCCGGCGTATGCCCCGGAATACCGCGCGGCGCATTCTTGC
CAAGATAGAGGAATATGCGCATGCGCCGGGTTCCCAGGCCAATAATGTGACCGCGCTGCGCGGGCGGGACGGGATACGCC
TCAGGGTCGGTGACTGGCGTGTGATCATGCAGGATGGTGAGGTGCTGGAGGTTTTGAAGATTGGCCCGCGTGGCGGGGTT
TATGACTAA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12124.01 Da
• Isoelectric Point: 5.1409

>AT122922 WP_135375597.1 NZ_CP038492:2926678-2927010 [Boseongicola sp. CCM32]
MAQTVTIPRIEYDALLSAREDLTDIRAYDAAMAAPEESLPHAFMLRLIEGVPPVLVFREWRGLSQAALARVSGVNRVQIS
DIEQRGKTGSVKTLRKLADALQVPLDDLVP

Nucleotide

• Length: 333 bp

>AT122922 NZ_CP038492:2926678-2927010 [Boseongicola sp. CCM32]
ATGGCGCAAACCGTAACAATCCCGCGTATCGAATATGATGCGCTGCTTTCCGCACGGGAGGATTTGACAGATATCCGTGC
CTATGATGCCGCCATGGCCGCGCCCGAGGAAAGCCTGCCGCATGCCTTCATGCTCCGGCTGATTGAAGGGGTGCCGCCGG
TTCTGGTGTTTCGGGAATGGCGCGGGCTCAGCCAGGCGGCGCTGGCCAGGGTTTCGGGTGTGAACCGGGTGCAGATCAGC
GATATCGAACAGCGCGGCAAGACCGGCTCGGTCAAAACGCTGAGAAAGCTTGCCGATGCGTTGCAGGTCCCGCTGGACGA
TCTGGTGCCCTGA