Detailed information of TA system TA122920

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 2129723..2130276
• Replicon: chromosome
• Accession: NZ_CP038492
• Organism: Boseongicola sp. CCM32

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: E2K80_RS10280
• Protein ID: WP_135374929.1
• Coordinates: 2130028..2130276 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: E2K80_RS10275
• Protein ID: WP_135374928.1
• Coordinates: 2129723..2130031 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E2K80_RS10250	2125111..2125581	+	471	WP_135374923.1	GNAT family N-acetyltransferase	-
E2K80_RS10255	2125615..2126298	+	684	WP_135374924.1	molecular chaperone DjiA	-
E2K80_RS10260	2126309..2127355	+	1047	WP_135374925.1	endonuclease/exonuclease/phosphatase family protein	-
E2K80_RS10265	2127387..2127740	+	354	WP_135374926.1	AAA+ family ATPase	-
E2K80_RS10270	2127886..2129736	+	1851	WP_135374927.1	M3 family oligoendopeptidase	-
E2K80_RS10275	2129723..2130031	-	309	WP_135374928.1	BrnA antitoxin family protein	Antitoxin
E2K80_RS10280	2130028..2130276	-	249	WP_135374929.1	BrnT family toxin	Toxin
E2K80_RS10285	2130649..2132304	-	1656	WP_135374930.1	tryptophan 7-halogenase	-
E2K80_RS10290	2132435..2132743	-	309	WP_135374931.1	DUF4389 domain-containing protein	-
E2K80_RS10295	2132969..2133388	-	420	WP_135374932.1	hypothetical protein	-
E2K80_RS10300	2133926..2134423	+	498	WP_135374933.1	DinB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (26-98)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9085.53 Da
• Isoelectric Point: 5.6388

>T122920 WP_135374929.1 NZ_CP038492:c2130276-2130028 [Boseongicola sp. CCM32]
MITIVWDEPKRQINLSRHGLDFADLDEWFFLDAVTVPAKEGRHMAIGCLSDGTIAVVFAVLGTEGVSVISMRPASRKERS
LL

Nucleotide

• Length: 249 bp

>T122920 NZ_CP038492:c2130276-2130028 [Boseongicola sp. CCM32]
ATGATAACAATAGTCTGGGATGAGCCGAAACGGCAGATCAACCTGTCTCGGCACGGGTTGGATTTTGCTGATCTGGACGA
ATGGTTCTTCCTTGATGCTGTGACGGTCCCCGCCAAGGAAGGCCGTCACATGGCAATAGGTTGCTTGAGCGATGGAACCA
TCGCCGTTGTCTTCGCTGTTCTGGGAACAGAAGGCGTTTCGGTGATCTCTATGCGTCCTGCAAGTCGCAAGGAAAGGAGT
TTGCTATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11223.79 Da
• Isoelectric Point: 10.3641

>AT122920 WP_135374928.1 NZ_CP038492:c2130031-2129723 [Boseongicola sp. CCM32]
MTAKKRTGISDAEEARIQKMIASDPDSPELTDDQIASAKPFSEGFPEIAQKMRKNIGGRPKSDSPKVPVSIRLDQDVIAK
FKATGPGWQSRINEVLRKSTVQ

Nucleotide

• Length: 309 bp

>AT122920 NZ_CP038492:c2130031-2129723 [Boseongicola sp. CCM32]
ATGACTGCCAAAAAGCGCACTGGAATTTCTGACGCTGAGGAAGCTCGCATCCAGAAGATGATCGCATCTGACCCTGACAG
CCCTGAACTGACAGATGACCAGATCGCCTCGGCCAAACCCTTTTCTGAGGGTTTCCCTGAGATTGCCCAGAAGATGCGTA
AGAACATCGGAGGGCGTCCAAAGTCGGACTCTCCAAAGGTGCCCGTGTCGATCCGACTGGATCAGGATGTTATTGCCAAA
TTCAAGGCAACTGGACCGGGCTGGCAGAGCCGTATTAATGAAGTGCTACGCAAGTCTACGGTGCAGTAA