Detailed information of TA system TA122909

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 2426068..2426647
• Replicon: chromosome
• Accession: NZ_CP038471
• Organism: Enterobacter roggenkampii strain BP10374

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A656ULU9
• Locus tag: E4005_RS11700
• Protein ID: WP_025912099.1
• Coordinates: 2426068..2426355 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: E4005_RS11705
• Protein ID: WP_039023943.1
• Coordinates: 2426342..2426647 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4005_RS11670	2421081..2421197	+	117	Protein_2216	amino acid-binding protein	-
E4005_RS11675	2421190..2421885	+	696	WP_032664591.1	winged helix-turn-helix domain-containing protein	-
E4005_RS11680	2422031..2422501	+	471	WP_008501325.1	MarR family transcriptional regulator	-
E4005_RS11685	2422498..2423565	+	1068	WP_085930304.1	HlyD family secretion protein	-
E4005_RS11690	2423555..2424631	+	1077	WP_023293361.1	DUF2955 domain-containing protein	-
E4005_RS11695	2424628..2425899	-	1272	WP_126348752.1	DUF445 domain-containing protein	-
E4005_RS11700	2426068..2426355	+	288	WP_025912099.1	BrnT family toxin	Toxin
E4005_RS11705	2426342..2426647	+	306	WP_039023943.1	BrnA antitoxin family protein	Antitoxin
E4005_RS11710	2426674..2427312	-	639	WP_135345793.1	LysE family translocator	-
E4005_RS11715	2427360..2428103	-	744	WP_021240616.1	AraC family transcriptional regulator	-
E4005_RS11720	2428255..2429625	+	1371	WP_135345794.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
E4005_RS11725	2429668..2429988	-	321	WP_008501314.1	hypothetical protein	-
E4005_RS11730	2429988..2430533	-	546	WP_008501313.1	porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11235.68 Da
• Isoelectric Point: 7.4007

>T122909 WP_025912099.1 NZ_CP038471:2426068-2426355 [Enterobacter roggenkampii]
MPTEYEWDSNKAKSNLQKHGIRFEDAVMVFDDPYHLSVQDRYENGEFRWQTIGLVQGLLVILVAHTVRFESGGEIIRIIS
ARKADRKERSRYEHR

Nucleotide

• Length: 288 bp

>T122909 NZ_CP038471:2426068-2426355 [Enterobacter roggenkampii]
ATGCCAACGGAGTATGAGTGGGATAGCAATAAGGCGAAAAGTAACCTGCAAAAACATGGCATACGCTTTGAGGATGCGGT
TATGGTTTTCGACGATCCTTATCATTTGTCTGTTCAGGATCGCTATGAGAACGGTGAATTTCGCTGGCAAACCATCGGCC
TTGTGCAAGGTCTTCTTGTTATTCTGGTCGCACATACGGTACGTTTTGAAAGCGGAGGAGAAATCATCCGTATCATTAGC
GCACGAAAGGCGGACCGCAAAGAGAGGAGCCGTTATGAGCATCGTTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11527.12 Da
• Isoelectric Point: 10.0592

>AT122909 WP_039023943.1 NZ_CP038471:2426342-2426647 [Enterobacter roggenkampii]
MSIVKHKRGSAPEVSARREAELKALAEKPEAEIDYSDIPATDDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKNK

Nucleotide

• Length: 306 bp

>AT122909 NZ_CP038471:2426342-2426647 [Enterobacter roggenkampii]
ATGAGCATCGTTAAACATAAACGCGGTAGTGCGCCTGAGGTAAGCGCTCGTCGTGAGGCTGAGCTCAAAGCATTAGCTGA
AAAACCAGAAGCAGAGATTGATTACAGCGATATCCCTGCTACGGACGATGAACAGTGGTCAGGGGCGGTGCGAGGAAAGT
TTTATCGTCCACTCAAAACTCAGGCATCTGTACGCATTGACGCCGATGTGATGGAGTGGCTTAAACGGCCTGGGAAAGGT
TACCAGACGCGGCTCAATGCTATTTTGCGAGAAGCGATGCTGCGCGATCAAAACAAAAATAAATAG

Structures

Toxin

• AlphaFold DB: A0A656ULU9