Detailed information of TA system TA122904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2166540..2167160
• Replicon: chromosome
• Accession: NZ_CP038469
• Organism: Citrobacter tructae strain SNU WT2

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: E4Z61_RS11230
• Protein ID: WP_002892050.1
• Coordinates: 2166942..2167160 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: E4Z61_RS11225
• Protein ID: WP_115259201.1
• Coordinates: 2166540..2166914 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4Z61_RS11215	2161693..2162883	+	1191	WP_135322832.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
E4Z61_RS11220	2162906..2166055	+	3150	WP_135322833.1	efflux RND transporter permease AcrB	-
E4Z61_RS11225	2166540..2166914	+	375	WP_115259201.1	Hha toxicity modulator TomB	Antitoxin
E4Z61_RS11230	2166942..2167160	+	219	WP_002892050.1	hemolysin expression modulator Hha	Toxin
E4Z61_RS11235	2167346..2167897	+	552	WP_135322834.1	maltose O-acetyltransferase	-
E4Z61_RS11240	2168014..2168484	+	471	WP_135322835.1	YlaC family protein	-
E4Z61_RS11245	2168558..2168698	-	141	WP_005125190.1	type B 50S ribosomal protein L36	-
E4Z61_RS11250	2168700..2168960	-	261	WP_135322836.1	type B 50S ribosomal protein L31	-
E4Z61_RS11255	2169147..2170700	+	1554	WP_135322837.1	EAL domain-containing protein	-
E4Z61_RS11260	2170764..2171117	-	354	WP_135322838.1	DUF1428 family protein	-
E4Z61_RS11265	2171200..2171811	-	612	WP_135322839.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T122904 WP_002892050.1 NZ_CP038469:2166942-2167160 [Citrobacter tructae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T122904 NZ_CP038469:2166942-2167160 [Citrobacter tructae]
ATGTCTGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGCTGCCAGACAATTGACACGCTTGAGCGCGT
TATTGAGAAAAATAAATATGAATTGTCCGACAATGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACGATGAATAAGCTCTACGACAAGATCCCAACTTCCGTTTGGAAATTTATACGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14454.26 Da
• Isoelectric Point: 5.5653

>AT122904 WP_115259201.1 NZ_CP038469:2166540-2166914 [Citrobacter tructae]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSNYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT122904 NZ_CP038469:2166540-2166914 [Citrobacter tructae]
ATGGACGAGTACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGCAACCATGGCTGGGTTAATGACCCAACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCACAGATTGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAATTATGGTATTAATACGCAGGATTTGCAGAAATGGCGAAAGTCCGGGAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2