Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2090177..2090706 Replicon chromosome
Accession NZ_CP038461
Organism Staphylococcus aureus strain O217

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag SaO217_RS10145 Protein ID WP_000621176.1
Coordinates 2090177..2090539 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag SaO217_RS10150 Protein ID WP_000948331.1
Coordinates 2090536..2090706 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SaO217_RS10125 2087155..2087925 - 771 WP_001041107.1 RNA polymerase sigma factor SigB -
SaO217_RS10130 2087900..2088379 - 480 WP_001190825.1 anti-sigma B factor RsbW -
SaO217_RS10135 2088381..2088707 - 327 WP_001052491.1 anti-sigma factor antagonist -
SaO217_RS10140 2088826..2089827 - 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
SaO217_RS10145 2090177..2090539 - 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
SaO217_RS10150 2090536..2090706 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
SaO217_RS10155 2090791..2091939 - 1149 WP_001281140.1 alanine racemase -
SaO217_RS10160 2092005..2092364 - 360 WP_000581197.1 holo-ACP synthase -
SaO217_RS10165 2092368..2092859 - 492 WP_001286801.1 PH domain-containing protein -
SaO217_RS10170 2092852..2094429 - 1578 WP_001294646.1 PH domain-containing protein -
SaO217_RS10175 2094422..2094901 - 480 WP_001287082.1 hypothetical protein -
SaO217_RS10180 2095110..2095670 - 561 WP_001092414.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T122865 WP_000621176.1 NZ_CP038461:c2090539-2090177 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T122865 NZ_CP038461:c2090539-2090177 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACATACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGTTCATCAGAAAAATTAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT122865 WP_000948331.1 NZ_CP038461:c2090706-2090536 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT122865 NZ_CP038461:c2090706-2090536 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References