Detailed information of TA system TA122849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2093854..2094383
• Replicon: chromosome
• Accession: NZ_CP038460
• Organism: Staphylococcus aureus strain B119

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SaO322_RS10810
• Protein ID: WP_000621175.1
• Coordinates: 2093854..2094216 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SaO322_RS10815
• Protein ID: WP_000948331.1
• Coordinates: 2094213..2094383 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SaO322_RS10785	2090832..2091602	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
SaO322_RS10790	2091577..2092056	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
SaO322_RS10795	2092058..2092384	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SaO322_RS10800	2092503..2093504	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SaO322_RS10810	2093854..2094216	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SaO322_RS10815	2094213..2094383	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SaO322_RS10820	2094468..2095616	-	1149	WP_001281150.1	alanine racemase	-
SaO322_RS10825	2095682..2096041	-	360	WP_000581198.1	holo-ACP synthase	-
SaO322_RS10830	2096045..2096536	-	492	WP_001286982.1	PH domain-containing protein	-
SaO322_RS10835	2096529..2098106	-	1578	WP_001294643.1	PH domain-containing protein	-
SaO322_RS10840	2098099..2098578	-	480	WP_001287084.1	hypothetical protein	-
SaO322_RS10845	2098787..2099347	-	561	WP_001092415.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T122849 WP_000621175.1 NZ_CP038460:c2094216-2093854 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T122849 NZ_CP038460:c2094216-2093854 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT122849 WP_000948331.1 NZ_CP038460:c2094383-2094213 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT122849 NZ_CP038460:c2094383-2094213 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1