122839

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 112171..112696 Replicon plasmid pEC129_2
Accession NZ_CP038455
Organism Escherichia coli strain EC-129

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag E4Z89_RS25315 Protein ID WP_001159871.1
Coordinates 112171..112476 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag E4Z89_RS25320 Protein ID WP_000813634.1
Coordinates 112478..112696 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4Z89_RS25285 107903..108928 + 1026 WP_000864945.1 zinc-binding alcohol dehydrogenase family protein -
E4Z89_RS25290 108987..109658 - 672 WP_046788564.1 FadR family transcriptional regulator -
E4Z89_RS25295 109753..110295 - 543 Protein_131 GGDEF domain-containing protein -
E4Z89_RS25300 110312..111009 - 698 Protein_132 IS1 family transposase -
E4Z89_RS25305 111061..111189 - 129 Protein_133 colicin M immunity protein -
E4Z89_RS25310 111361..112170 - 810 WP_000016963.1 site-specific integrase -
E4Z89_RS25315 112171..112476 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
E4Z89_RS25320 112478..112696 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
E4Z89_RS25335 113164..113859 + 696 WP_072652234.1 DNA-binding protein -
E4Z89_RS25340 113856..114476 + 621 WP_072652229.1 hypothetical protein -
E4Z89_RS25345 114473..115261 + 789 WP_072652230.1 DUF2971 domain-containing protein -
E4Z89_RS25350 115297..116037 + 741 WP_072652231.1 abortive infection family protein -
E4Z89_RS25355 116196..116825 - 630 Protein_141 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / dfrA27 / aadA16 / sul1 / rmtB / blaTEM-1B / aac(3)-IId / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / tet(A) - 1..117599 117599


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T122839 WP_001159871.1 NZ_CP038455:c112476-112171 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T122839 NZ_CP038455:c112476-112171 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT122839 WP_000813634.1 NZ_CP038455:c112696-112478 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT122839 NZ_CP038455:c112696-112478 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References