Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 112171..112696 Replicon plasmid pEC129_2
Accession NZ_CP038455
Organism Escherichia coli strain EC-129

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag E4Z89_RS25315 Protein ID WP_001159871.1
Coordinates 112171..112476 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag E4Z89_RS25320 Protein ID WP_000813634.1
Coordinates 112478..112696 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4Z89_RS25285 107903..108928 + 1026 WP_000864945.1 zinc-binding alcohol dehydrogenase family protein -
E4Z89_RS25290 108987..109658 - 672 WP_046788564.1 FadR family transcriptional regulator -
E4Z89_RS25295 109753..110295 - 543 Protein_131 GGDEF domain-containing protein -
E4Z89_RS25300 110312..111009 - 698 Protein_132 IS1 family transposase -
E4Z89_RS25305 111061..111189 - 129 Protein_133 colicin M immunity protein -
E4Z89_RS25310 111361..112170 - 810 WP_000016963.1 site-specific integrase -
E4Z89_RS25315 112171..112476 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
E4Z89_RS25320 112478..112696 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
E4Z89_RS25335 113164..113859 + 696 WP_072652234.1 DNA-binding protein -
E4Z89_RS25340 113856..114476 + 621 WP_072652229.1 hypothetical protein -
E4Z89_RS25345 114473..115261 + 789 WP_072652230.1 DUF2971 domain-containing protein -
E4Z89_RS25350 115297..116037 + 741 WP_072652231.1 abortive infection family protein -
E4Z89_RS25355 116196..116825 - 630 Protein_141 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / dfrA27 / aadA16 / sul1 / rmtB / blaTEM-1B / aac(3)-IId / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / tet(A) - 1..117599 117599


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T122839 WP_001159871.1 NZ_CP038455:c112476-112171 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T122839 NZ_CP038455:c112476-112171 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT122839 WP_000813634.1 NZ_CP038455:c112696-112478 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT122839 NZ_CP038455:c112696-112478 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References