Detailed information of TA system TA122828

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3671339..3672033
• Replicon: chromosome
• Accession: NZ_CP038453
• Organism: Escherichia coli strain EC-129

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: E4Z89_RS17740
• Protein ID: WP_001094398.1
• Coordinates: 3671339..3671707 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: E4Z89_RS17745
• Protein ID: WP_077249034.1
• Coordinates: 3671728..3672033 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4Z89_RS17720	3666512..3667468	+	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-
E4Z89_RS17725	3667465..3669663	+	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
E4Z89_RS17730	3669673..3670629	+	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
E4Z89_RS17735	3670608..3671018	+	411	WP_001111348.1	hypothetical protein	-
E4Z89_RS17740	3671339..3671707	-	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
E4Z89_RS17745	3671728..3672033	-	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
E4Z89_RS17750	3672137..3672781	-	645	WP_000086755.1	hypothetical protein	-
E4Z89_RS17755	3672800..3673021	-	222	WP_000692345.1	DUF987 domain-containing protein	-
E4Z89_RS17760	3673090..3673566	-	477	WP_001424026.1	RadC family protein	-
E4Z89_RS17765	3673582..3674055	-	474	WP_000855059.1	antirestriction protein	-
E4Z89_RS17775	3674397..3675218	-	822	WP_001234565.1	DUF945 domain-containing protein	-
E4Z89_RS17785	3675319..3675527	-	209	Protein_3436	DUF905 family protein	-
E4Z89_RS17790	3675628..3676083	-	456	WP_000581506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagX/ecpC / yagW/ecpD / yagV/ecpE	3644850..3672781	27931

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T122828 WP_001094398.1 NZ_CP038453:c3671707-3671339 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T122828 NZ_CP038453:c3671707-3671339 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
AACTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT122828 WP_077249034.1 NZ_CP038453:c3672033-3671728 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT122828 NZ_CP038453:c3672033-3671728 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4