Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1869370..1870205 Replicon chromosome
Accession NZ_CP038453
Organism Escherichia coli strain EC-129

Toxin (Protein)


Gene name yeeV Uniprot ID A0A7X1VUT4
Locus tag E4Z89_RS08950 Protein ID WP_025693544.1
Coordinates 1869370..1869747 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID Q5I3J0
Locus tag E4Z89_RS08955 Protein ID WP_001280951.1
Coordinates 1869837..1870205 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4Z89_RS08910 1864890..1865363 + 474 WP_001105407.1 DNA gyrase inhibitor SbmC -
E4Z89_RS08915 1865561..1866619 + 1059 WP_001200895.1 FUSC family protein -
E4Z89_RS08920 1866791..1867120 + 330 WP_000450409.1 DUF496 family protein -
E4Z89_RS08925 1867221..1867403 - 183 WP_024174802.1 ethanolamine utilization protein -
E4Z89_RS08935 1867857..1868885 - 1029 Protein_1728 IS110 family transposase -
E4Z89_RS08940 1869044..1869157 - 114 WP_032140621.1 DUF957 domain-containing protein -
E4Z89_RS08945 1869170..1869373 - 204 WP_000761705.1 hypothetical protein -
E4Z89_RS08950 1869370..1869747 - 378 WP_025693544.1 TA system toxin CbtA family protein Toxin
E4Z89_RS08955 1869837..1870205 - 369 WP_001280951.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
E4Z89_RS08960 1870368..1870589 - 222 WP_000692286.1 DUF987 domain-containing protein -
E4Z89_RS08965 1870652..1871128 - 477 WP_001186747.1 RadC family protein -
E4Z89_RS08970 1871144..1871617 - 474 WP_000855059.1 antirestriction protein -
E4Z89_RS08980 1871959..1872777 - 819 WP_086714495.1 DUF945 domain-containing protein -
E4Z89_RS08990 1872932..1873090 - 159 WP_001323397.1 DUF905 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - galF / cpsACP / wzi / wza / gnd / wbtL / ugd / fyuA 1681644..1914813 233169


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14039.01 Da        Isoelectric Point: 7.2919

>T122816 WP_025693544.1 NZ_CP038453:c1869747-1869370 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SAGASSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Download         Length: 378 bp

>T122816 NZ_CP038453:c1869747-1869370 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13699.56 Da        Isoelectric Point: 7.0268

>AT122816 WP_001280951.1 NZ_CP038453:c1870205-1869837 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT122816 NZ_CP038453:c1870205-1869837 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7X1VUT4

Antitoxin

Source ID Structure
AlphaFold DB Q5I3J0

References