Detailed information of TA system TA122814

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1337005..1337630
• Replicon: chromosome
• Accession: NZ_CP038453
• Organism: Escherichia coli strain EC-129

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: E4Z89_RS06435
• Protein ID: WP_000911330.1
• Coordinates: 1337232..1337630 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: E4Z89_RS06430
• Protein ID: WP_000450524.1
• Coordinates: 1337005..1337232 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4Z89_RS06405	1332808..1333278	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
E4Z89_RS06410	1333278..1333850	-	573	WP_137088594.1	glycine cleavage system transcriptional repressor	-
E4Z89_RS06415	1333996..1334874	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
E4Z89_RS06420	1334891..1335925	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
E4Z89_RS06425	1336138..1336851	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
E4Z89_RS06430	1337005..1337232	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
E4Z89_RS06435	1337232..1337630	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
E4Z89_RS06440	1337777..1338640	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
E4Z89_RS06445	1338655..1340670	+	2016	WP_001402395.1	tRNA cytosine(34) acetyltransferase TmcA	-
E4Z89_RS06450	1340744..1341442	+	699	WP_000679812.1	esterase	-
E4Z89_RS06455	1341552..1341752	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T122814 WP_000911330.1 NZ_CP038453:1337232-1337630 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T122814 NZ_CP038453:1337232-1337630 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT122814 WP_000450524.1 NZ_CP038453:1337005-1337232 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT122814 NZ_CP038453:1337005-1337232 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33