Detailed information of TA system TA122809

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 55241..55934
• Replicon: plasmid pAeme6
• Accession: NZ_CP038450
• Organism: Aeromonas media strain R50-22

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: E4188_RS23080
• Protein ID: WP_000182276.1
• Coordinates: 55241..55597 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: N2IIN5
• Locus tag: E4188_RS23085
• Protein ID: WP_001172026.1
• Coordinates: 55599..55934 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4188_RS23065	50622..51182	-	561	WP_171277332.1	hypothetical protein	-
E4188_RS23070	51434..54463	-	3030	WP_032432545.1	Tn3 family transposase	-
E4188_RS23075	54447..55049	-	603	WP_010465829.1	recombinase family protein	-
E4188_RS23080	55241..55597	+	357	WP_000182276.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4188_RS23085	55599..55934	+	336	WP_001172026.1	helix-turn-helix domain-containing protein	Antitoxin
E4188_RS23090	55949..56284	+	336	WP_000741275.1	hypothetical protein	-
E4188_RS23095	56308..56634	+	327	WP_000091614.1	hypothetical protein	-
E4188_RS23100	56631..57011	+	381	WP_001054412.1	hypothetical protein	-
E4188_RS23105	57266..58381	+	1116	WP_171277333.1	HD-GYP domain-containing protein	-
E4188_RS23110	58480..58782	+	303	WP_088813953.1	IS66 family insertion sequence hypothetical protein	-
E4188_RS23115	58782..59147	+	366	WP_041205730.1	IS66 family insertion sequence element accessory protein TnpB	-
E4188_RS23120	59196..60839	+	1644	WP_171277334.1	IS66 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(E) / msr(E) / tet(E) / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr	-	1..199818	199818
	-	inside	IScluster/Tn	mph(E) / msr(E) / tet(E) / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr	-	45157..94015	48858

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-93)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12912.93 Da
• Isoelectric Point: 8.9018

>T122809 WP_000182276.1 NZ_CP038450:55241-55597 [Aeromonas media]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKELKGFGGAGVLKVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQEL

Nucleotide

• Length: 357 bp

>T122809 NZ_CP038450:55241-55597 [Aeromonas media]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGAGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGAAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12201.39 Da
• Isoelectric Point: 10.6058

>AT122809 WP_001172026.1 NZ_CP038450:55599-55934 [Aeromonas media]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Nucleotide

• Length: 336 bp

>AT122809 NZ_CP038450:55599-55934 [Aeromonas media]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A024EKI7