Detailed information of TA system TA122804

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3681766..3683480
• Replicon: chromosome
• Accession: NZ_CP038444
• Organism: Aeromonas media strain T5-8

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: E4186_RS17045
• Protein ID: WP_171269281.1
• Coordinates: 3682827..3683480 (-)
• Length: 218 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: E4186_RS17040
• Protein ID: WP_171269501.1
• Coordinates: 3681766..3682830 (-)
• Length: 355 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4186_RS17025	3676890..3677828	+	939	WP_171269279.1	restriction endonuclease	-
E4186_RS17030	3677847..3678560	-	714	WP_005331303.1	M48 family metallopeptidase	-
E4186_RS17035	3678560..3681691	-	3132	WP_171269280.1	HsdR family type I site-specific deoxyribonuclease	-
E4186_RS17040	3681766..3682830	-	1065	WP_171269501.1	macro domain-containing protein	Antitoxin
E4186_RS17045	3682827..3683480	-	654	WP_171269281.1	DUF4433 domain-containing protein	Toxin
E4186_RS17050	3683747..3685123	-	1377	WP_171269282.1	restriction endonuclease subunit S	-
E4186_RS17055	3685123..3687570	-	2448	WP_171269283.1	type I restriction-modification system subunit M	-
E4186_RS17060	3687756..3688202	-	447	WP_021138009.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3672750..3709046	36296

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-216)

Antitoxin

• Macro domain (18-131)

Sequences

Toxin

Protein

• Length: 218 a.a.
• Molecular weight: 25486.15 Da
• Isoelectric Point: 7.0177

>T122804 WP_171269281.1 NZ_CP038444:c3683480-3682827 [Aeromonas media]
MAYDYSQSLNPQKALIFRIVHRDNLEWILTHGLWAGNGQQQNPHWVHIGLPELIAKRARHPVPLVPYGVLNDYVPFYFTP
FSPMLSNIHSGRGVPRRENDEILILVSSLHRMAELGLPFVFTDSHAYYQWANFYVDLGDLERIDWPLLQRRDFKRDAEDL
SKLERYQAEALIHQHCPIEALLGVVCYNEEIKNRVEQCMNRVGVARPVYVRDGWYFR

Nucleotide

• Length: 654 bp

>T122804 NZ_CP038444:c3683480-3682827 [Aeromonas media]
ATGGCCTATGACTATAGCCAGAGCCTGAATCCGCAGAAGGCGCTGATATTCCGGATCGTGCATCGGGATAACCTGGAATG
GATCCTGACCCATGGCCTGTGGGCGGGGAATGGTCAGCAGCAAAACCCGCACTGGGTGCATATCGGGTTGCCGGAGCTGA
TTGCCAAGCGCGCGCGTCATCCGGTGCCGCTGGTGCCTTACGGCGTGTTGAATGACTATGTGCCCTTCTATTTCACGCCG
TTTTCTCCCATGCTCAGCAATATTCACTCGGGGCGCGGTGTTCCCCGGCGTGAAAATGATGAAATCCTGATCCTGGTTTC
CAGCCTGCATCGCATGGCTGAATTGGGGCTACCGTTTGTGTTTACCGATAGCCATGCCTATTACCAGTGGGCCAATTTCT
ATGTCGATCTTGGCGATCTGGAGCGGATTGACTGGCCACTGTTGCAGCGCCGTGATTTCAAGCGTGATGCGGAAGATTTG
AGCAAGCTGGAACGCTATCAGGCCGAGGCCTTGATCCATCAACATTGCCCCATCGAGGCGTTGCTGGGCGTGGTGTGTTA
CAACGAAGAGATAAAGAACCGCGTGGAACAGTGCATGAATCGGGTCGGGGTCGCGCGCCCCGTCTATGTCCGTGATGGGT
GGTATTTCAGATGA

Antitoxin

Protein

• Length: 355 a.a.
• Molecular weight: 39645.80 Da
• Isoelectric Point: 6.8636

>AT122804 WP_171269501.1 NZ_CP038444:c3682830-3681766 [Aeromonas media]
MIRYTTGNLLDAPAEALVNTVNTVGVMGKGIALMFKERFPANMRAYAEACKQGDVVTGKMFITQTGELLGPRWIVNFPTK
QHWRNPAQMAWIQEGLQDLRAWLQAQQVSSIAIPPLGAGNGGLPWLEVKQAIETALGDLDIDILLYEPSRQYQNVAKRGG
VAELTPARALIAELVRRYWFLGMECSLLEIQKLAWFLARVIGRNPLGDPLQLQFKAHRYGPYANNLTHLLNAMDGSYLQS
DKRIPDSKPFDVIWFNEAKKQELAVYLQTEAKAFLPILEQTTALIDGFESPYGMELLATVDWLIAEEGCAPTVSALREGL
HRWPAGTPWGERKARLFDDRSLQIALTRLQQGLV

Nucleotide

• Length: 1065 bp

>AT122804 NZ_CP038444:c3682830-3681766 [Aeromonas media]
ATGATTCGATATACAACAGGCAATCTGCTGGATGCTCCGGCTGAAGCTCTGGTGAATACCGTCAACACCGTCGGTGTCAT
GGGCAAGGGGATCGCCTTGATGTTCAAGGAGCGGTTCCCGGCCAATATGCGTGCCTATGCCGAGGCGTGCAAGCAGGGTG
ACGTCGTCACCGGCAAGATGTTTATTACCCAGACGGGTGAGCTGCTGGGGCCACGCTGGATCGTGAATTTTCCGACCAAA
CAGCACTGGCGCAATCCGGCACAGATGGCGTGGATCCAGGAGGGTTTGCAGGATCTGCGGGCCTGGTTACAGGCGCAGCA
GGTCTCCTCGATTGCCATTCCACCACTGGGGGCTGGCAACGGTGGTCTGCCTTGGCTGGAGGTCAAACAGGCGATTGAAA
CGGCACTGGGCGATCTGGATATCGATATCCTGCTCTATGAGCCCAGTCGCCAATACCAAAATGTGGCCAAGCGTGGCGGG
GTGGCGGAGCTGACTCCGGCTCGGGCCTTGATTGCTGAACTGGTACGTCGCTACTGGTTTTTAGGGATGGAGTGCAGCTT
GCTGGAAATCCAGAAGCTGGCCTGGTTTCTGGCTCGGGTCATTGGCCGCAACCCGCTGGGGGATCCGCTGCAGTTGCAGT
TTAAGGCCCATCGTTATGGCCCTTATGCCAACAATCTGACTCATCTGCTCAATGCGATGGACGGCAGTTATCTGCAGTCC
GACAAGCGCATTCCGGATAGCAAGCCGTTTGATGTCATCTGGTTTAATGAGGCCAAAAAACAAGAGCTTGCCGTTTATCT
CCAGACCGAGGCCAAAGCATTTTTACCGATACTGGAGCAGACGACCGCGTTAATCGATGGTTTTGAGTCGCCCTATGGCA
TGGAGTTATTGGCCACGGTCGATTGGTTGATAGCCGAAGAGGGCTGTGCGCCGACGGTGAGCGCGCTGCGAGAGGGGTTG
CATCGCTGGCCAGCGGGAACCCCGTGGGGTGAGCGCAAGGCGCGGCTGTTTGACGATCGCAGTCTGCAGATCGCCTTGAC
CCGTTTGCAGCAGGGATTGGTGTAA