Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 5347421..5349147 Replicon chromosome
Accession NZ_CP038438
Organism Pseudomonas fluorescens strain LBUM677

Toxin (Protein)


Gene name pmenT Uniprot ID -
Locus tag E4T63_RS24695 Protein ID WP_096796317.1
Coordinates 5347421..5348065 (+) Length 215 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID -
Locus tag E4T63_RS24700 Protein ID WP_135296644.1
Coordinates 5348062..5349147 (+) Length 362 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4T63_RS24675 5342709..5343914 - 1206 WP_135296641.1 restriction endonuclease subunit S -
E4T63_RS24680 5343911..5345449 - 1539 WP_135296642.1 type I restriction-modification system subunit M -
E4T63_RS24685 5345737..5346657 + 921 WP_135296643.1 restriction endonuclease -
E4T63_RS24690 5346843..5347424 + 582 WP_098965796.1 DUF4433 domain-containing protein -
E4T63_RS24695 5347421..5348065 + 645 WP_096796317.1 DUF4433 domain-containing protein Toxin
E4T63_RS24700 5348062..5349147 + 1086 WP_135296644.1 macro domain-containing protein Antitoxin
E4T63_RS24705 5349321..5349881 + 561 WP_135296645.1 hypothetical protein -
E4T63_RS24710 5349897..5350331 + 435 WP_135296646.1 hypothetical protein -
E4T63_RS24715 5350355..5351167 - 813 WP_041064922.1 DUF2135 domain-containing protein -
E4T63_RS24720 5351171..5352790 - 1620 WP_135296647.1 DUF2300 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 5337644..5349147 11503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(14-213)

Antitoxin

Macro domain

(18-130)


Sequences

Toxin        


Download         Length: 215 a.a.        Molecular weight: 24904.56 Da        Isoelectric Point: 6.9118

>T122796 WP_096796317.1 NZ_CP038438:5347421-5348065 [Pseudomonas fluorescens]
MIYTNLNPEKALIWRIVHRDNLPWILDNGLHCANSEAKAPQYVNIGNVDLIDKRRFRQVPIAPEGVLADYVPFYFTPFSV
MMQNIHSGWSVQQRGNDEIVILVSSLYRVEELGLPFVFTNAHAYPSWTNYYSDLANLDQIDWSILQRRDFKRDPDDPRKM
ERYQAEALIHQHLPITGLLGIMCHTDAMKVRIEADVAVRGLTLPVHARPKWYFQ

Download         Length: 645 bp

>T122796 NZ_CP038438:5347421-5348065 [Pseudomonas fluorescens]
ATGATCTATACCAACCTGAATCCAGAGAAAGCTCTGATTTGGCGTATCGTCCATCGCGATAACCTGCCCTGGATTCTGGA
CAACGGTCTGCATTGCGCAAACTCGGAAGCAAAGGCACCTCAGTACGTGAATATCGGCAACGTCGACTTGATCGACAAAC
GCCGTTTCCGTCAGGTGCCTATCGCACCCGAAGGCGTACTGGCCGACTACGTGCCCTTCTATTTCACACCCTTCTCGGTG
ATGATGCAGAACATTCACTCTGGCTGGAGTGTTCAACAACGCGGCAATGACGAGATCGTGATTCTGGTCTCAAGCCTCTA
TCGCGTTGAAGAGCTTGGCCTACCGTTTGTCTTCACAAACGCTCATGCCTACCCGAGTTGGACAAACTACTACAGCGATC
TGGCGAATCTCGACCAGATCGATTGGTCTATCCTTCAACGGCGCGACTTCAAGCGCGATCCTGACGACCCTCGCAAAATG
GAGCGTTATCAGGCTGAGGCGCTGATCCACCAACATTTACCGATTACGGGACTACTGGGCATCATGTGCCACACCGATGC
AATGAAAGTACGCATAGAAGCAGACGTGGCCGTTAGAGGCTTGACGTTGCCGGTCCATGCGCGTCCCAAATGGTATTTCC
AATGA

Antitoxin


Download         Length: 362 a.a.        Molecular weight: 40568.67 Da        Isoelectric Point: 6.7603

>AT122796 WP_135296644.1 NZ_CP038438:5348062-5349147 [Pseudomonas fluorescens]
MIRFTQGNLLEAKTEALVNTVNTVGVMGKGIALMFKERFAQNYRVYAAACKAGEVETGKMHVTATNELDGPRWIVNFPTK
RHWRSPSQMAWITEGLHDLRRFLLENDVKSVAVPPLGAGNGGLKWPEVREQIIEVLGDLDVDVLVFEPSNQYLNVAKRDG
VEQLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERAIEKIPGTENPLNLKFVAHKFGPYANRLEHLLNNLDGSYLH
CDKRISDAGITDVIWFDQDRKAFLQTYLKTEAKEYSQALERTAELIDGFESPFGMELLATVDWLLIRERIAPTVPAVRKA
LKNWDGGSGAAVRKNKLFDDQALGIAITRLTSSSFSPELAA

Download         Length: 1086 bp

>AT122796 NZ_CP038438:5348062-5349147 [Pseudomonas fluorescens]
ATGATCAGATTCACCCAAGGCAACCTGCTGGAAGCCAAAACTGAAGCGCTCGTCAATACGGTGAATACCGTGGGCGTGAT
GGGTAAGGGCATCGCGCTGATGTTCAAAGAGCGCTTTGCGCAAAATTACCGCGTGTATGCAGCGGCATGTAAGGCCGGGG
AAGTAGAAACCGGCAAGATGCACGTGACGGCCACCAATGAACTCGATGGCCCACGCTGGATCGTGAACTTCCCTACCAAA
CGCCATTGGCGTTCACCGTCGCAAATGGCGTGGATAACTGAAGGCTTGCATGATCTGCGTCGGTTTCTGCTTGAGAACGA
CGTGAAATCTGTCGCCGTCCCACCTCTCGGTGCTGGTAACGGCGGTTTGAAGTGGCCTGAAGTACGCGAGCAGATTATAG
AAGTGCTGGGTGACCTGGATGTCGATGTATTAGTGTTCGAACCCTCTAATCAGTATCTGAATGTCGCTAAGCGCGATGGC
GTTGAGCAACTCACTCCGGCACGCGCACTGATTGCTGAGTTGGTCCGGCGTTATTGGGTTCTGGGCATGGAATGCAGCCT
GCTCGAAATTCAGAAACTGGCTTGGTTTCTTGAGCGTGCCATCGAGAAAATTCCAGGTACCGAGAATCCGCTAAACCTCA
AATTTGTCGCTCATAAATTCGGTCCGTATGCCAATCGATTGGAGCATCTGCTCAACAATCTGGATGGCAGCTATCTACAT
TGCGACAAGCGCATCAGTGATGCAGGAATCACCGATGTCATTTGGTTTGATCAAGATCGCAAAGCCTTTTTGCAAACCTA
CCTCAAGACTGAAGCCAAGGAATACTCCCAGGCTTTAGAGCGAACGGCTGAATTGATCGACGGTTTTGAGTCACCTTTCG
GCATGGAGTTGCTGGCCACCGTTGATTGGTTGCTGATTCGGGAAAGGATTGCTCCAACCGTTCCTGCGGTACGTAAAGCA
CTGAAGAACTGGGACGGTGGGTCAGGAGCTGCGGTGCGTAAAAACAAGTTATTCGATGATCAGGCACTGGGAATCGCGAT
CACTCGTCTGACATCCAGCAGTTTCAGCCCGGAACTAGCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References