Detailed information of TA system TA122791

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1794461..1795077
• Replicon: chromosome
• Accession: NZ_CP038438
• Organism: Pseudomonas fluorescens strain LBUM677

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A854WZU8
• Locus tag: E4T63_RS08165
• Protein ID: WP_027613935.1
• Coordinates: 1794461..1794673 (+)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: E4T63_RS08170
• Protein ID: WP_115987135.1
• Coordinates: 1794673..1795077 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4T63_RS08140	1790564..1791100	+	537	WP_135295231.1	DsbE family thiol:disulfide interchange protein	-
E4T63_RS08145	1791097..1791567	+	471	WP_003222948.1	cytochrome c-type biogenesis protein CcmH	-
E4T63_RS08150	1791564..1792766	+	1203	WP_135295232.1	c-type cytochrome biogenesis protein CcmI	-
E4T63_RS08155	1792795..1793199	+	405	WP_135295233.1	hypothetical protein	-
E4T63_RS08160	1793306..1794193	-	888	WP_135295234.1	LysR family transcriptional regulator	-
E4T63_RS08165	1794461..1794673	+	213	WP_027613935.1	type II toxin-antitoxin system HicA family toxin	Toxin
E4T63_RS08170	1794673..1795077	+	405	WP_115987135.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
E4T63_RS08175	1795136..1796323	-	1188	WP_135295235.1	MFS transporter	-
E4T63_RS08180	1796567..1797187	+	621	WP_134785766.1	hypothetical protein	-
E4T63_RS08185	1797467..1798744	+	1278	WP_135295236.1	hypothetical protein	-
E4T63_RS08190	1798731..1799678	+	948	WP_127646222.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 8013.21 Da
• Isoelectric Point: 10.3701

>T122791 WP_027613935.1 NZ_CP038438:1794461-1794673 [Pseudomonas fluorescens]
VQSRLLIKELEEAGWTLDRVTGSHHIFTHRYNPYTIPVPHPKKDLPLGTVKSIRRRAGLYHPPASYKGDP

Nucleotide

• Length: 213 bp

>T122791 NZ_CP038438:1794461-1794673 [Pseudomonas fluorescens]
GTGCAAAGCAGGCTATTGATCAAGGAGCTGGAGGAGGCAGGCTGGACGCTGGATCGAGTCACCGGCAGTCATCACATCTT
CACGCATCGTTACAACCCGTACACCATTCCCGTTCCTCACCCTAAAAAGGATTTGCCGCTGGGGACGGTCAAAAGCATCA
GGAGGCGTGCCGGGTTGTACCACCCGCCAGCCAGCTACAAGGGAGATCCATAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14818.77 Da
• Isoelectric Point: 4.2870

>AT122791 WP_115987135.1 NZ_CP038438:1794673-1795077 [Pseudomonas fluorescens]
MQYPICIEWGDDNTAIGIQIPDIPGAVTAGDTFEDAYNAAVEVAHLMLQEIAADGESIPMPTSAAAHRNNPEFADMGWGM
LELDISPYMGKTEKVNVTLPGYVIQRIDRYVREHNVKSRSSFLADAAMEKLVRY

Nucleotide

• Length: 405 bp

>AT122791 NZ_CP038438:1794673-1795077 [Pseudomonas fluorescens]
ATGCAATATCCGATCTGCATCGAATGGGGTGACGACAACACCGCCATCGGTATTCAGATACCTGACATTCCCGGCGCCGT
CACGGCCGGGGACACTTTCGAGGACGCCTATAACGCAGCCGTTGAAGTCGCCCATCTCATGTTGCAGGAGATAGCGGCGG
ACGGGGAATCGATTCCGATGCCGACGTCGGCAGCGGCCCATCGCAATAATCCGGAGTTTGCCGACATGGGCTGGGGGATG
CTCGAGCTGGATATCTCGCCGTACATGGGCAAGACCGAGAAGGTCAACGTGACGTTGCCAGGCTATGTGATCCAGCGCAT
CGATCGCTATGTGCGTGAACACAATGTCAAAAGCCGCTCTTCGTTTCTGGCAGATGCGGCGATGGAGAAGCTGGTTCGGT
ATTAA

Structures

Toxin

• AlphaFold DB: A0A854WZU8