122777

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4073588..4074219 Replicon chromosome
Accession NZ_CP038428
Organism Escherichia coli O157:H7 strain 611

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag E3149_RS20710 Protein ID WP_001259386.1
Coordinates 4073944..4074219 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag E3149_RS20705 Protein ID WP_000593555.1
Coordinates 4073588..4073947 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3149_RS20675 4068700..4069173 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
E3149_RS20680 4069170..4069451 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
E3149_RS20685 4069528..4070886 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
E3149_RS20690 4070879..4072387 + 1509 WP_001301773.1 carbohydrate kinase -
E3149_RS20695 4072377..4072646 + 270 WP_000933060.1 HPr family phosphocarrier protein -
E3149_RS20700 4072678..4073538 + 861 WP_001131788.1 class II aldolase -
E3149_RS20705 4073588..4073947 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
E3149_RS20710 4073944..4074219 - 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
E3149_RS20715 4074292..4075416 - 1125 WP_001301659.1 ABC transporter permease -
E3149_RS20720 4075416..4078151 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
E3149_RS20725 4078148..4079215 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T122777 WP_001259386.1 NZ_CP038428:c4074219-4073944 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T122777 NZ_CP038428:c4074219-4073944 [Escherichia coli O157:H7]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCGCTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT122777 WP_000593555.1 NZ_CP038428:c4073947-4073588 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT122777 NZ_CP038428:c4073947-4073588 [Escherichia coli O157:H7]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References