Detailed information of TA system TA122761

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1988203..1988674
• Replicon: chromosome
• Accession: NZ_CP038428
• Organism: Escherichia coli O157:H7 strain 611

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: E3149_RS10310
• Protein ID: WP_001303511.1
• Coordinates: 1988396..1988674 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: E3149_RS10305
• Protein ID: WP_001302048.1
• Coordinates: 1988203..1988394 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3149_RS10265	1983410..1983994	-	585	WP_000206793.1	DUF551 domain-containing protein	-
E3149_RS10270	1984051..1984446	-	396	WP_001118159.1	DUF977 family protein	-
E3149_RS10275	1984462..1985231	-	770	Protein_2016	DUF1627 domain-containing protein	-
E3149_RS10280	1985257..1985997	-	741	WP_000788938.1	ATP-binding protein	-
E3149_RS10285	1986004..1986966	-	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
E3149_RS10290	1986989..1987414	-	426	WP_000693943.1	Rha family transcriptional regulator	-
E3149_RS10295	1987411..1987713	-	303	WP_000172738.1	transcriptional regulator	-
E3149_RS10300	1987811..1988182	+	372	WP_001169686.1	hypothetical protein	-
E3149_RS10305	1988203..1988394	+	192	WP_001302048.1	hypothetical protein	Antitoxin
E3149_RS10310	1988396..1988674	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E3149_RS10315	1989027..1989326	+	300	WP_001240334.1	hypothetical protein	-
E3149_RS10320	1989398..1989616	+	219	WP_001171930.1	hypothetical protein	-
E3149_RS10325	1989620..1989784	-	165	WP_001331716.1	hypothetical protein	-
E3149_RS10330	1990185..1990373	+	189	WP_000449192.1	cell division inhibition protein DicB	-
E3149_RS10335	1990370..1990561	+	192	WP_001090200.1	DUF1482 family protein	-
E3149_RS10340	1990654..1993125	+	2472	WP_000048458.1	exonuclease	-
E3149_RS10345	1993198..1993449	+	252	WP_000005552.1	excisionase family protein	-
E3149_RS10350	1993484..1993668	+	185	Protein_2031	DUF3596 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1950391..1996961	46570

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T122761 WP_001303511.1 NZ_CP038428:1988396-1988674 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T122761 NZ_CP038428:1988396-1988674 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT122761 WP_001302048.1 NZ_CP038428:1988203-1988394 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT122761 NZ_CP038428:1988203-1988394 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• AlphaFold DB: A0A829CTP0